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Catalog Number | orb752954 |
---|---|
Category | Proteins |
Description | IL-10 Human produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 166 amino acids (19-178 aa) and having a molecular mass of 19.4kDa. IL-10 is fused to a 6 amino acid His tag at C-terminus and purified by proprietary chromatographic techniques. |
Form/Appearance | Sterile Filtered colorless solution. |
Purity | Greater than 95.0% as determined by SDS-PAGE. |
Protein Sequence | SPGQGTQSEN SCTHFPGNLP NMLRDLRDAF SRVKTFFQMK DQLDNLLLKE SLLEDFKGYL GCQALSEMIQ FYLEEVMPQA ENQDPDIKAH VNSLGENLKT LRLRLRRCHR FLPCENKSKA VEQVKNAFNK LQEKGIYKAM SEFDIFINYI EAYMTMKIRN HHHHHH |
Source | Sf9, Baculovirus cells |
Biological Activity | Measured in a cell proliferation assay using MC/9 mouse mast cells. The ED50 range < 5 ng/ml. |
Storage | Stability: Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles |
Buffer/Preservatives | The IL-10 solution (0.5mg/ml) contains 10% Glycerol and Phosphate-Buffered Saline (pH 7.4). |
Alternative names | Interleukin-10, IL-10, Cytokine synthesis inhibito Read more... |
Note | For research use only |
Application notes | Cytokines And Growth Factors |
Expiration Date | 6 months from date of receipt. |
> 95% as determined by SDS-PAGE and HPLC. | |
18.6 kDa | |
E.Coli |
The purity of the protein is greater than 85% as determined by SDS-PAGE and Coomassie blue staining. | |
The protein has a predicted molecular mass of 19.5 kDa after removal of the signal peptide. The apparent molecular mass of His-IL10 is approximately 15-25 kDa due to glycosylation. | |
Mammalian |
The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining. | |
The protein has a predicted molecular mass of 44.8 kDa after removal of the signal peptide. The apparent molecular mass of hFc-IL10 is approximately 35-55 kDa due to glycosylation. | |
Mammalian |
ELISA, MS, SDS-PAGE, WB | |
Greater than 95% as determined by reducing SDS-PAGE. | |
Human cells |