Androgen Receptor Antibody

SKU: orb18847

Description

Goat polyclonal antibody to AR

Images & Validation

• Tested Applications: ChIP, ELISA, IF, IHC, WB
• Dilution range: ELISA: 1:64000, WB: 0.1-0.5 μg/ml, IHC-P: 2-3 μg/ml
• Reactivity: Human
• Predicted Reactivity: Bovine, Canine, Mouse, Porcine, Rat
• Application Notes:
  ELISA: Peptide ELISA: antibody detection limit dilution 1:64000.WB: Approx 95kDa band observed in Human Brain and Human Heart lysates (calculated MW of 99kDa according to NP_000035). Recommended concentration: 0.3-2 μg/ml.

Key Properties

• Host: Goat
• Clonality: Polyclonal
• Immunogen: Peptide with sequence EVQLGLGRVYPRPPSC, from the N Terminus of the protein sequence
• Target: Androgen Receptor
• Molecular Weight: Approx 100kDa band observed in Human Cerebellum lysates (calculated MW of 99.2kDa)
• Purity: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin
• Disclaimer: For research use only

Alternative Names

anti androgen receptor antibody, anti dihydrotestosterone receptor antibody, anti AR antibody, anti KD antibody, anti AIS antibody, anti TFM antibody, anti DHTR antibody, anti SBMA antibody, anti NR3C4 antibody, anti SMAX1 antibody, anti HUMARA antibody, anti HYSP1 antibody, anti androgen receptor antibody, anti dihydrotestosterone receptor antibody, anti RP11-383C12.1 antibody

staining of Human Cerebellum (A) and (0.1 μg/ml) negative control Pancreas (B) lysate (35 μg protein in RIPA buffer). Detected by chemiluminescence.

2 μg/ml staining of paraffin embedded Human Prostate. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

ChIP of 2 μg with 1 μg DHT-treated HEC50 chromatin using the spin column sonication kit (Protein G) measuring FKBP5 enrichment.

Immunofluorescence analysis of paraformaldehyde fixed MCF7 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 μg/ml) followed by Alexa Fluor 488 secondary antibody (2 μg/ml), showing Mitochondrial/cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 μg/ml) followed by Alexa Fluor 488 secondary antibody (2 μg/ml).

Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 μg/ml) followed by Alexa Fluor 488 secondary antibody (2 μg/ml), showing Mitochondrial/cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 μg/ml) followed by Alexa Fluor 488 secondary antibody (2 μg/ml).

Immunostaining of 25μm thick cryosections of PFA-perfused Mouse Hypothalamus, antigen retrieval with citrate buffer Ph 6 at 80°C for 30 min, HRP-staining with Ni-DAB after Biotin-SP-antigoat amplification.

Immunostaining of 25μm thick cryosections of PFA-perfused Mouse Amygdala, antigen retrieval with citrate buffer Ph 6 at 80°C for 30 min, HRP-staining with Ni-DAB after Biotin-SP-antigoat amplification.

Quick Database Links

Gene Symbol

Androgen Receptor

NCBI Gene ID

367