GFAP antibody

SKU: orb10706

Description

GFAP antibody

Images & Validation

• Tested Applications: ELISA, ICC, IF, IHC-P, WB
• Dilution range: WB: 1:100-1000, IHC-P: 1:100-1000, IF/ICC: 1:100-1000 (based on 0.5mg/ml)
• Reactivity: Human, Mouse, Rat

Key Properties

• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human GFAP. Please contact us for the exact immunogen sequence. The peptide is available as orb374725.
• Target: GFAP
• Molecular Weight: 50 kDa
• Purity: Polyclonal antibodies are purified by peptide affinity chromatography
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: 10 mM PBS, 0.02% sodium azide
• Concentration: - 100 μg (in 200 μl): 0.5 mg/ml- 200 μg (in 400 μl): 0.5 mg/ml
• Disclaimer: For research use only

Alternative Names

anti-Glial Fibrillary Acidic Protein antibody, anti-Astrocyte antibody, anti-FLJ45472 antibody, anti-GFAP antibody, anti-Glial Fibrillary Acidic Protein antibody, anti-Intermediate filament protein antibody

Western blot analysis of rat brain (lane 1), rat spinal cord (lane 2), rat lung (lane 3), mouse pancreas (lane 4), rat liver (lane 5), U251 cells (lane 6) using GFAP antibody (1 ug/ml)

Immunofluorescence analysis of mouse lung tissue using GFAP antibody (2.5 ug/ml)

Immunohistochemical staining of paraffin embedded rat pancreas tissue using GFAP antibody (2.5 ug/ml)

Immunohistochemical staining of paraffin embedded mouse brain tissue using GFAP antibody (2.5 ug/ml)

Immunohistochemical staining of rat pancreas tissue using anti-GFAP (2.5 ug/ml)

IHC-P staining of mouse brain tissue using GFAP antibody (2.5 ug/ml)

Immunohistochemical staining of paraffin embedded mouse lung tissue using anti-GFAP (2.5 ug/ml)

Immunohistochemical staining of paraffin embedded rat spinal cord tissue using GFAP antibody (2.5 ug/ml)

IHC-P staining of mouse skin tissue using anti-GFAP (2.5 ug/ml)

IHC-P image of mouse skin tissue using GFAP antibody (2.5 ug/ml)

IHC-P staining of rat pancreas tissue using anti-GFAP (2.5 ug/ml)

IF image of mouse lung tissue using GFAP antibody (2.5 ug/ml)

Immunohistochemical staining of mouse brain tissue using anti-GFAP (2.5 ug/ml)

Immunohistochemical staining of paraffin embedded mouse lung tissue using anti-GFAP (2.5 ug/ml)

WB analysis of rat brain (lane 1), rat spinal cord (lane 2), rat lung (lane 3), mouse pancreas (lane 4), rat liver (lane 5), U251 cells (lane 6) using GFAP antibody (1 ug/ml)

Quick Database Links

Gene Symbol

GFAP

UniProt

Q28115, P14136, P03995

NCBI Gene ID

2670

References

Masoumeh Rahmatinia 1, Anoushiravan Mohseni-Bandpei 1, Fariba Khodagholi 2, Mohammad-Amin Abdollahifar 3, Mehdi Amouei Torkmahalleh 4, Meysam Hassani Moghaddam 5, Philip K Hopke 6, Ehsan Ghavimehr 7, Shahriyar Bazzazpour 8, Abbas Shahsavani 9 Exposure to different PM2.5 extracts induces gliosis and changes behavior in male rats similar to autism spectrum disorders features Environ Pollut, 340, 122804 (2024)

PubMed: 37907193

Applications

IHC

Reactivity

Rat

Zahra Behroozi # 1, Behnaz Rahimi # 2, Ali Motamednezhad # 3, Alireza Ghadaksaz 4, Zeinab Hormozi-Moghaddam 5 6, Ali Moshiri 7, Maral Jafarpour 8, Pooya Hajimirzaei 5 6, Ali Ataie 9, Atousa Janzadeh Combined effect of Cerium oxide nanoparticles loaded scaffold and photobiomodulation therapy on pain and neuronal regeneration following spinal cord injury: an experimental study Photochem Photobiol Sci ., (2024)

PubMed: 38300466

Applications

WB

Reactivity

Rat