General 25-Hydroxyvitamin D3 (HVD3) ELISA Kit

• Catalog Number: orb1806532
• Category: Assays and Kits
• Description: General 25-Hydroxyvitamin D3 (HVD3) ELISA Kit
• Target: HVD3
• Reactivity: All
• Application notes: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with 25-Hydroxyvitamin D3(HVD3) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to 25-Hydroxyvitamin D3(HVD3). Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of 25-Hydroxyvitamin D3(HVD3) in the samples is then determined by comparing the OD of the samples to the standard curve.
• Assay Type: Competitive
• Assay Time: 1.5H
• Standard: 200ng/mL
• Range: 3.13-200ng/mL
• Sensitivity: 0.91 ng/mL
• Sample Types: serum, plasma and other biological fluids
• Alternative names: 25-HVD3; 25HVD3; Calcifediol; Calcidiol; H-VD3; 25
• Research Area: Metabolism Research, Musculoskeletal & Connective
• Note: For research use only
• Expiration Date: Please enquire.