DUSP6 Antibody (Center)

SKU: orb1928492

Description

DUSP6 Antibody (Center)

Images & Validation

• Tested Applications: IF, IHC-P, WB
• Dilution range: WB - 1:500
• Reactivity: Human, Mouse, Rat
• Predicted Reactivity: Bovine

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Molecular Weight: 42320 Da

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Disclaimer: For research use only

Alternative Names

MKP3, PYST1

Confocal immunofluorescent analysis of DUSP6 Antibody (Center) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).

All lanes: Anti-DUSP6 Antibody (Center) at 1:2000 dilution. Lane 1: Human brain lysate. Lane 2: Human lung lysate. Lane 3: Rat brain lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 42 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

All lanes: Anti-DUSP6 Antibody (Center) at 1:2000 dilution. Lane 1: HepG2 whole cell lysate. Lane 2: Human brain tissue lysate. Lane 3: Rat brain tissue lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 42 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

All lanes: Anti-DUSP6 Antibody (Center) at 1:500 dilution. Lane 1: Human brain tissue lysate. Lane 2: Mouse brain tissue lysate. Lane 3: Rat brain tissue lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 42 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Immunohistochemical analysis of paraffin-embedded human kidney tissue was performed on the Leica BOND RXm. Tissue was fixed with formaldehyde at room temperature, antigen retrieval was by heat mediation with a EDTA buffer (pH9.0). Samples were incubated with primary antibody (1:500) for 1 hours at room temperature. A undiluted biotinylated CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.

Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue was performed on the Leica BOND RXm. Tissue was fixed with formaldehyde at room temperature, antigen retrieval was by heat mediation with a EDTA buffer (pH9.0). Samples were incubated with primary antibody (1:500) for 1 hours at room temperature. A undiluted biotinylated CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.

Quick Database Links

UniProt

Q16828