CD44 Antibody

SKU: orb44252

Description

Mouse Monoclonal to CD44.

Images & Validation

• Tested Applications: FC, IHC-P, IP, WB
• Reactivity: Canine, Human, Porcine
• Application Notes:
  Flow cytometry: Recommended dilution: 4 μg/ml. Western blotting: Recommended dilution: 2 μg/ml, 60 min on vertical incubator; positive control: Kg-1a human acute leukemia cell lysate, JURKAT human leukemia T-cell lysate, non-reducing conditions, 6% separating gel. Immunohistochemistry (paraffin sections): Recommended dilution: 10 μg/ml; positive tissue: uterus, myometrium.

Key Properties

• Antibody Type: Primary Antibody
• Clonality: Monoclonal
• Isotype: Mouse IgG1
• Clone No.: MEM-263
• Immunogen: COS-7 cells (African Green Monkey).
• Target: CD44
• Purification: Purified by protein-A affinity chromatography.

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Concentration: 1 mg/ml
• Disclaimer: For research use only

Alternative Names

PGP-I, HUTCH-I, ECMR-III, Hermes antigen, Hyaluronate receptor, Heparan sulfate proteoglycan, Epican, MC56, MIC4, INLU, LHR

Western blotting analysis (non-reducing conditions) of isolated peripheral blood lymphocytes of various species using anti-CD44 (MEM-263). Lane 1: lysate of human PBL; Lane 2: lysate of canine PBL; Lane 3: lysate of porcine PBL.

Western blotting analysis of CD44 in MOLT-4 cells (lane 1) and HeLa cells (lane 2) using anti-CD44 (MEM-263) purified; non-reducing conditions.

Separation of human CD44 positive lymphocytes (red-filled) from CD44 negative blood debris (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD44 (MEM-263) purified antibody (concentration in sample 4 µg/ml) GAM APC.

Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD44 (MEM-263) purified antibody (concentration in sample 4 µg/ml) GAM APC.

Quick Database Links

Gene Symbol

CD44

UniProt

P16070

NCBI Gene ID

960