Anti-c-myc epitope tag [9E10]

• Catalog Number: orb256352
• Category: Antibodies
• Description: Human monoclonal antibody to c-myc tag
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: 9E10
• Tested applications: IF, IHC-Fr, IHC-P, IP, WB
• Reactivity: Human
• Isotype: Mouse IgG2
• Immunogen: A synthetic peptide corresponding to aa 408-439 from C-terminus of human c-myc.
• Concentration: 1 mg/ml
• Purity: Purified
• Conjugation: Unconjugated
• Target: c-myc epitope tag
• UniProt ID: P01106
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% Proclin 300.
• Alternative names: Proto-oncogene c-Myc; Transcription factor p64; Cl
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

ELISA of anti-epitope tag antibodies on multi-tag protein Plate coated with 5 ug/ml of Multitag protein. Primary antibody loaded at 1000 ng/ml in first well and an 11 point serial dilution performed in duplicate for each antibody with last well left blank. Signal detection was performed using HRP-conjugated goat anti-mouse.

Left Panel: orb256348 (2 µg/ml) staining of HEK293 spiked (A) and unspiked (B) with 5 ng multitag protein (35 µg protein in RIPA buffer). Lane C contains 25 ng purified multitag protein as positive control. Right Panel: 1 µg/ml staining of Multitag protein at 25 ng (A), 10 ng (B) and 5 ng (C) amounts. For both, primary incubation was 1 hour. Detected by chemiluminescence.