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Catalog Number | orb345103 |
---|---|
Category | Antibodies |
Description | IL6 antibody |
Species/Host | Mouse |
Clonality | Monoclonal |
Clone Number | 33A12.G9 |
Tested applications | ELISA, FC, Multiplex Assay, WB |
Reactivity | Human |
Isotype | IgG1 |
Immunogen | This Protein A purified monoclonal antibody was produced in mouse by repeated immunizations with mature full length recombinant human IL-6 produced in E.coli followed by hybridoma development. |
Concentration | 1.0 mg/mL |
Dilution range | ELISA: 1:10,000, FC: 0.5 mg/mL, WB: 1:1000 |
Form/Appearance | Liquid (sterile filtered) |
Purity | This purified antibody detects recombinant and native IL-6 present in body fluids and cell supernatants in various assays (ie. IL-1 stimulated IL-6 production from fibroblasts). In Western blot analysis of natural cell products or human body fluids, multiple bands of IL-6 will appear due to the variable amount of glycosylation on the molecule. |
Conjugation | Unconjugated |
UniProt ID | P05231 |
NCBI | NP_000591.1 |
Storage | Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing. |
Buffer/Preservatives | 0.01% (w/v) Sodium Azide |
Alternative names | mouse Anti-IL-6 antibody, mouse anti-interleukin-6 Read more... |
Note | For research use only |
Application notes | Anti-IL 6 antibody has been tested for use in ELISA, Flow Cytometry, and western blotting. Reactivity is also expected in neutralizations, radioimmunoassay and immunohistochemistry. The endotoxin content is estimated to be < 10 pg/µl by the LAL method. By western blot a band approximately 23.7 kDa in size corresponding to native human IL-6 protein is expected in the appropriate cell lysate or extract. Specific conditions for reactivity should be optimized by the end user. |
Expiration Date | 12 months from date of receipt. |
Western blot analysis of detection of Human IL-6. 100 ng of Human IL-6 was run on a 4-20% gel and transferred to 0.45 um nitrocellulose using IL6 antibody
Flow cytometric analysis of Figure A: un-stimulated, Figure B: 1 ug/mL LPS in a protein transport inhibitor for 5 hours Staining: (surface) x-axis:(intracellular) y-axis: using IL6 antibody
Flow cytometric analysis of human PBMCs were stimulated with 1ug/mL LPS and a transport inhibitor for 4-5 hours. Cells were then suspended in fixation buffer for 10-12 minutes and vortexed briefly using IL6 antibody
ELISA, IF, IHC-Fr, IHC-P | |
Human | |
Rabbit | |
Polyclonal | |
Unconjugated |
Unconjugated | |
95% | |
39.4 kDa | |
Human IL-6 R alpha, His Tag (orb257625) is expressed from human 293 cells (HEK293). It contains AA Leu 20 - Pro 365 (Accession # NP_000556.1). |
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