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IL6 antibody

Catalog Number: orb345103

DispatchUsually dispatched within 5-10 working days
$ 292.00
Catalog Numberorb345103
CategoryAntibodies
DescriptionIL6 antibody
Species/HostMouse
ClonalityMonoclonal
Clone Number33A12.G9
Tested applicationsELISA, FC, Multiplex Assay, WB
ReactivityHuman
IsotypeIgG1
ImmunogenThis Protein A purified monoclonal antibody was produced in mouse by repeated immunizations with mature full length recombinant human IL-6 produced in E.coli followed by hybridoma development.
Concentration1.0 mg/mL
Dilution rangeELISA: 1:10,000, FC: 0.5 mg/mL, WB: 1:1000
Form/AppearanceLiquid (sterile filtered)
PurityThis purified antibody detects recombinant and native IL-6 present in body fluids and cell supernatants in various assays (ie. IL-1 stimulated IL-6 production from fibroblasts). In Western blot analysis of natural cell products or human body fluids, multiple bands of IL-6 will appear due to the variable amount of glycosylation on the molecule.
ConjugationUnconjugated
UniProt IDP05231
NCBINP_000591.1
StorageStore vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
Buffer/Preservatives0.01% (w/v) Sodium Azide
Alternative namesmouse Anti-IL-6 antibody, mouse anti-interleukin-6
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NoteFor research use only
Application notesAnti-IL 6 antibody has been tested for use in ELISA, Flow Cytometry, and western blotting. Reactivity is also expected in neutralizations, radioimmunoassay and immunohistochemistry. The endotoxin content is estimated to be < 10 pg/µl by the LAL method. By western blot a band approximately 23.7 kDa in size corresponding to native human IL-6 protein is expected in the appropriate cell lysate or extract. Specific conditions for reactivity should be optimized by the end user.
Expiration Date12 months from date of receipt.
IL6 antibody

Western blot analysis of detection of Human IL-6. 100 ng of Human IL-6 was run on a 4-20% gel and transferred to 0.45 um nitrocellulose using IL6 antibody

IL6 antibody

Flow cytometric analysis of Figure A: un-stimulated, Figure B: 1 ug/mL LPS in a protein transport inhibitor for 5 hours Staining: (surface) x-axis:(intracellular) y-axis: using IL6 antibody

IL6 antibody

Flow cytometric analysis of human PBMCs were stimulated with 1ug/mL LPS and a transport inhibitor for 4-5 hours. Cells were then suspended in fixation buffer for 10-12 minutes and vortexed briefly using IL6 antibody

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