IL6 antibody

• Catalog Number: orb345102
• Category: Antibodies
• Description: IL6 antibody
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: 33A12.G9
• Tested applications: ELISA, FC, Multiplex Assay, WB
• Reactivity: Human
• Isotype: IgG1
• Immunogen: This Protein A purified IL-6 monoclonal antibody was produced in mouse by repeated immunizations with mature full length recombinant human IL-6 produced in E.coli followed by hybridoma development.
• Concentration: 1.0 mg/mL
• Dilution range: ELISA: 1:10,000, FC: 0.5 mg/mL, WB: 1:1000
• Form/Appearance: Liquid (sterile filtered)
• Purity: This purified antibody detects recombinant and native IL-6 present in body fluids and cell supernatants in various assays (ie. IL-1 stimulated IL-6 production from fibroblasts). In Western blot analysis of natural cell products or human body fluids, multiple bands of IL-6 will appear due to the variable amount of glycosylation on the molecule.
• Conjugation: Unconjugated
• UniProt ID: P05231
• NCBI: NP_000591.1
• Storage: Store IL 6 antibody at -20° C . For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Alternative names: mouse Anti-IL-6 antibody, mouse anti-interleukin-6
• Note: For research use only
• Application notes: Anti-IL-6 antibody has been tested for use in ELISA, Flow Cytometry, and western blotting. Reactivity is also expected in neutralizations, radioimmunoassay and immunohistochemistry. The endotoxin content is estimated to be < 10 pg/µl by the LAL method. By western blot a band approximately 23.7 kDa in size corresponding to native human IL-6 protein is expected in the appropriate cell lysate or extract. Specific conditions for reactivity should be optimized by the end user.
• Expiration Date: 12 months from date of receipt.

Western blot analysis of detection of Human IL-6. 100 ng of Human IL-6 was run on a 4-20% gel and transferred to 0.45 um nitrocellulose using IL6 antibody

Flow cytometric analysis of Figure A: un-stimulated, Figure B: 1 ug/mL LPS in a protein transport inhibitor for 5 hours Staining: (surface) x-axis: (intracellular) y-axis: using IL6 antibody

Flow cytometric analysis of human PBMCs were stimulated with 1ug/mL LPS and a transport inhibitor for 4-5 hours. Cells were then suspended in fixation buffer for 10-12 minutes and vortexed briefly using IL6 antibody