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Catalog Number | orb1262210 |
---|---|
Category | Antibodies |
Description | IL2RG Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | FC, IF, WB |
Reactivity | Human |
Isotype | Rabbit Ig |
Immunogen | This IL2RG antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 76-101 amino acids from the N-terminal region of human IL2RG. |
Antibody Type | Primary Antibody |
Concentration | batch dependent |
Form/Appearance | Liquid |
Conjugation | Unconjugated |
MW | 42 kDa |
Target | IL2RG |
UniProt ID | P31785 |
NCBI | P31785 |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Buffer/Preservatives | Supplied in PBS with 0.09% (W/V) sodium azide. |
Alternative names | Cytokine receptor common subunit gamma, Interleuki Read more... |
Note | For research use only |
Application notes | For IF starting dilution is: 1:25For WB starting dilution is: 1:1000For FACS starting dilution is: 1:10~50 |
Expiration Date | 12 months from date of receipt. |
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized human spleen tissue, followed by Alexa Fluor 488-conjugated goat anti-rabbit IgG secondary antibody at 1/400 dilution (green). Confocal image showing membrane staining on human spleen tissue. The nuclear counter stain is DAPI (blue).
Western Blot at 1:2000 dilution Lane 1: human spleen lysates Lane 2: Jurkat whole cell lysates Lane 3: Daudi whole cell lysates Lane 4: KG-1 whole cell lysates Lane 5: MOLT-4 whole cell lysates Lane 6: HL-60 whole cell lysates Lysates/proteins at 20 ug per lane.
Western blot analysis of lysate from Jurkat cell line, using IL2RG Antibody at 1:1000.
Western blot analysis of IL2RG Antibody in HL-60 cell line lysates (35 ug/lane)
Flow cytometric analysis of HL-60 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
FC, ICC | |
Bovine, Canine, Mouse, Porcine, Rat | |
Human | |
Rabbit | |
Polyclonal | |
Unconjugated |