IL2RG Antibody

• Catalog Number: orb1262210
• Category: Antibodies
• Description: IL2RG Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, IF, WB
• Reactivity: Human
• Isotype: Rabbit Ig
• Immunogen: This IL2RG antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 76-101 amino acids from the N-terminal region of human IL2RG.
• Concentration: batch dependent
• Dilution range: For IF starting dilution is: 1:25For WB starting dilution is: 1:1000For FACS starting dilution is: 1:10~50
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 42 kDa
• Target: IL2RG
• UniProt ID: P31785
• NCBI: P31785
• Storage: Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
• Buffer/Preservatives: Supplied in PBS with 0.09% (W/V) sodium azide.
• Alternative names: Cytokine receptor common subunit gamma, Interleuki
• Note: For research use only
• Application notes: For IF starting dilution is: 1:25For WB starting dilution is: 1:1000For FACS starting dilution is: 1:10~50
• Expiration Date: 12 months from date of receipt.

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized human spleen tissue, followed by Alexa Fluor 488-conjugated goat anti-rabbit IgG secondary antibody at 1/400 dilution (green). Confocal image showing membrane staining on human spleen tissue. The nuclear counter stain is DAPI (blue).

Western Blot at 1:2000 dilution Lane 1: human spleen lysates Lane 2: Jurkat whole cell lysates Lane 3: Daudi whole cell lysates Lane 4: KG-1 whole cell lysates Lane 5: MOLT-4 whole cell lysates Lane 6: HL-60 whole cell lysates Lysates/proteins at 20 ug per lane.

Western blot analysis of lysate from Jurkat cell line, using IL2RG Antibody at 1:1000.

Western blot analysis of IL2RG Antibody in HL-60 cell line lysates (35 ug/lane)

Flow cytometric analysis of HL-60 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.