IL2RG Antibody (N-term)

• Catalog Number: orb1927910
• Category: Antibodies
• Description: Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Clone Number: RB22117
• Tested applications: FC, IF, WB
• Reactivity: Human
• Isotype: Rabbit IgG
• Antibody Type: Primary Antibody
• Dilution range: IF: 1:25, WB: 1:1000, WB: 1:2000, WB: 1:1000, FC: 1:10~50
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Conjugation: Unconjugated
• MW: 42287 Da
• Target: This IL2RG antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 76-101 amino acids from the N-terminal region of human IL2RG.
• UniProt ID: P31785
• NCBI: NP_000197.1
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: Cytokine receptor common subunit gamma, Interleuki
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of IL2RG Antibody (N-term) in HL-60 cell line lysates (35 ug/lane). IL2RG (arrow) was detected using the purified Pab.

IL2RG Antibody (N-term) flow cytometric analysis of HL-60 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Western blot analysis of lysate from Jurkat cell line, using IL2RG Antibody (N-term). Diluted at 1:1000. A goat anti-rabbit IgG H&L (HRP) at 1:10000 dilution was used as the secondary antibody. Lysate at 20 ug.

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized human spleen tissue labeling IL2RG at 1/25 dilution, followed by Alexa Fluor 488-conjugated goat anti-rabbit IgG (1583138) secondary antibody at 1/400 dilution (green). Confocal image showing membrane staining on human spleen tissue. The nuclear counter stain is DAPI (blue).

All lanes: Anti-IL2RG Antibody (N-term) at 1:2000 dilution. Lane 1: human spleen lysates. Lane 2: Jurkat whole cell lysates. Lane 3: Daudi whole cell lysates. Lane 4: KG-1 whole cell lysates. Lane 5: MOLT-4 whole cell lysates. Lane 6: HL-60 whole cell lysates. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 42 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.