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Product Overview
Product Name IL1 beta antibody
Catalog Number orb10903
Species/HostRabbit
ReactivityHuman, Mouse, Rat
Predicted Reactivity Canine, Porcine
ConjugationUnconjugated
Tested applicationsELISA, FC, IF, IHC-P, WB
Immunogen KLH conjugated synthetic peptide derived between 111-190 amino acids of human IL1 beta
Target IL1 beta
Alternative Names
Product Properties
Form/Appearance Liquid: 0.01M TBS (pH7.4), 0.5% BSA, 0.015% ProClin™ 300 and 25% glycerol
Concentration - 5 μg (in 100 μl): 0.05 mg/ml
- 20 μg (in 100 μl): 0.2 mg/ml
- 100 μg (in 200 μl): 0.5 mg/ml
- 200 μg (in 400 μl): 0.5 mg/ml
Storage Store at 4°C for up to two weeks. For long term storage, aliquot and store at -20°C, avoid freeze/thaw cycles.
Note For research use only.
Isotype IgG
ClonalityPolyclonal
Purity Affinity purified by Protein A
MW 17 kDa
Uniprot ID P01584 (Human); P10749 (Mouse); Q63264 (Rat)
NCBI NP_000567.11; NM_000576.21
Entrez 3553
Product Description

Rabbit polyclonal antibody to IL1 beta

Application Notes
Dilution Range WB: 1:500-2000, ELISA: 1:500-1000, IHC-P: 1:400-800, Flow-Cyt: 1μg/Test, IF: 1:100-500
Validation Images
WB analysis of rat lung tissue using IL1 beta antibody (dilution of primary antibody - 1:5000)
WB analysis of rat lung tissue using IL1 beta antibody (dilution of primary antibody - 1:5000)
Immunohistochemical analysis of formalin fixed and paraffin embedded rat lung tissue using IL1 beta antibody
Immunohistochemical analysis of formalin fixed and paraffin embedded rat lung tissue using IL1 beta antibody
IHC-P image of mouse skin tissue using anti-IL1 beta (dilution of primary antibody at 1:100)
IHC-P image of mouse skin tissue using anti-IL1 beta (dilution of primary antibody at 1:100)
Immunohistochemical analysis of paraffin-embedded colon tissue of rat using IL1 beta antibody
Immunohistochemical analysis of paraffin-embedded colon tissue of rat using IL1 beta antibody
Immunohistochemical staining of rat brain tissue using IL1 beta antibody
Immunohistochemical staining of rat brain tissue using IL1 beta antibody
Flow cytometric analysis of A431 cells using IL1 beta antibody
Flow cytometric analysis of A431 cells using IL1 beta antibody
Immunohistochemical staining of Human cervical carcinoma tissue using IL1 beta antibody
Immunohistochemical staining of Human cervical carcinoma tissue using IL1 beta antibody
Western blot analysis of Mouse Raw246.7 Cell Lysate using IL1 beta antibody
Western blot analysis of Mouse Raw246.7 Cell Lysate using IL1 beta antibody
Additional Documents
References

NLRP3 Inflammasome Is Involved in Calcium-sensing receptor-induced Aortic Remodeling in SHRs

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