IL-2 Antibody

• Catalog Number: orb2635385
• Category: Antibodies
• Description: Interleukin-2 (IL-2) is a T cell stimulatory cytokine that induces T cell proliferation and NK cell proliferation and activation. Produced by T-cells in response to antigenic or mitogenic stimulation, this protein is required for T-cell proliferation and other activities crucial to regulation of the immune response. Can stimulate B-cells, monocytes, lymphokine-activated killer cells, natural killer cells, and glioma cells. The receptor of this cytokine is a heterotrimeric protein complex whose gamma chain is also shared by interleukin 4 (IL4) and interleukin 7 (IL7). IL-2 induces CTLA-4 and also functions as a survival factor for lymphocytes.
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: IL2/3949
• Tested applications: IHC-P
• Reactivity: Human
• Isotype: Mouse IgG2a, kappa
• Immunogen: A portion of amino acids 21-153 was used as the immunogen for the IL-2 antibody.
• Antibody Type: Primary Antibody
• Dilution range: Immunohistochemistry (FFPE): 1-2ug/ml
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
• Hazard Information: This IL-2 antibody is available for research use only.
• UniProt ID: P60568
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Note: For research use only
• Application notes: Optimal dilution of the IL-2 antibody should be determined by the researcher.
• Expiration Date: 12 months from date of receipt.

IHC staining of FFPE human adrenal gland with IL-2 antibody (clone IL2/3949). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human colon carcinoma tissue with IL-2 antibody (clone IL2/3949). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

SDS-PAGE analysis of purified, BSA-free IL-2 antibody (clone IL2/3949) as confirmation of integrity and purity.

Analysis of HuProt (TM) microarray containing more than 19000 full-length human proteins using IL-2 antibody (clone IL2/3949). These results demonstrate the foremost specificity of the IL2/3949 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt (TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt (TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.