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IgG2b Isotype control (PerCP)

Catalog Number: orb154540

DispatchUsually dispatched within 5-10 working days weeks.
$ 355.00
Catalog Numberorb154540
CategoryAntibodies
DescriptionMouse monoclonal antibody conjugated to PerCP which recognizes IgG2b that belongs to many immunoglobulin G. It can be used as a negative control, to distinguish specific from non-specific binding of mouse IgG2b fluorochrome-conjugated antibodies to human cells. It was found that IgG2a was superior to IgG1 in activating complement. IgG2 is an antibody moleculer induced by infection.
Species/HostMouse
ClonalityMonoclonal
Clone NumberMPC-11
Tested applicationsFC
IsotypeMouse IgG2b kappa
ImmunogenKLH-coupled trinitrophenol
Concentration0.1 mg/ml
Dilution rangeNegative control: The reagent is intended as an isotype control to establish the amount of non-specific antibody binding. For your particular experiment, use the same concentration of this control antibody as the recommended working concentration of the antigen-specific antibody. Also, when working with prediluted antibodies, dilute the isotype control to the same concentration as is the concentration of the antigen-specific antibody in the prediluted antibody solution you are using. If under particular experimental conditions the background signal of the isotype control is too high (usually when working concentrations of used antibodies are above 10 μg/ml of incubation mixture), change the conditions of your experiment to reduce the background.
PurityPurified antibody is conjugated with activated Peridinin-Chlorophyll Protein (PerCP) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
ConjugationPerCP
StorageStore at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Buffer/PreservativesStabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
NoteFor research use only
Application notesNegative control: The reagent is intended as an isotype control to establish the amount of non-specific antibody binding. For your particular experiment, use the same concentration of this control antibody as the recommended working concentration of the antigen-specific antibody. Also, when working with prediluted antibodies, dilute the isotype control to the same concentration as is the concentration of the antigen-specific antibody in the prediluted antibody solution you are using. If under particular experimental conditions the background signal of the isotype control is too high (usually when working concentrations of used antibodies are above 10 μg/ml of incubation mixture), change the conditions of your experiment to reduce the background.
Expiration Date12 months from date of receipt.
IgG2b Isotype control (PerCP)

Flow cytometric analysis of human peripheral blood using IgG2b Isotype control (PerCP)

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