IBA1 Antibody / AIF-1

• Catalog Number: orb606561
• Category: Antibodies
• Description: AIF1 (Allograft inflammatory factor 1), also called IBA1 (Ionized calcium-binding adapter molecule 1) is a cytoplasmic, calcium-binding protein that is thought to play a role in macrophage activation and function. AIF1/IBA1, containing two EF domains, is induced by cytokines and Interferons. In an unstimulated state, it colocalizes with actin, and upon stimulation, translocates to lamellipodia. It is also a marker of human microglia and is expressed by macrophages in injured skeletal muscle. The gene encoding the protein resides in the tumor necrosis factor (TNF) cluster of genes, located in the region represented by the human major histocompatibility complex (MHC).
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: AIF1/2493
• Tested applications: IHC-P
• Reactivity: Human
• Isotype: Mouse IgG2c, kappa
• Immunogen: A portion of amino acids 1-146 from the human protein was used as the immunogen for the AIF1 antibody.
• Antibody Type: Primary Antibody
• Dilution range: Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
• Purity: Protein G affinity chromatography
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
• Hazard Information: This AIF1 antibody is available for research use only.
• UniProt ID: P55008
• Storage: Store the AIF1 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide
• Note: For research use only
• Application notes: Titering of the AIF1 antibody may be required for optimal performance.
• Expiration Date: 12 months from date of receipt.

IHC testing of FFPE human kidney tissue with AIF1 antibody (clone AIF1/2493). HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.

IHC testing of FFPE human spleen tissue with AIF1 antibody (clone AIF1/2493). HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.

SDS-PAGE analysis of purified, BSA-free AIF1 antibody (clone AIF1/2493) as confirmation of integrity and purity.