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Product Overview
Product Name Human VEGFA protein (Active)
Catalog Number orb359168
ReactivityHuman
ConjugationUnconjugated
Sequence Partial of Isoform VEGF165
Endotoxins Less than 1.0 EU/μg as determined by LAL method.
Target VEGFA
Product Properties
Form/Appearance Lyophilized: 0.2 μm filtered PBS, pH 7.4
Storage Store at -20°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Tag Tag-Free
Note For research use only.
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°/-80°. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Protein Sequence M+APMAEGGGQ NHHEVVKFMD VYQRSYCHPI ETLVDIFQEY PDEIEYIFKP SCVPLMRCGG CCNDEGLECV PTEESNITMQ IMRIKPHQGQ HIGEMSFLQH NKCECRPKKD RARQENPCGP CSERRKHLFV QDPQTCKCSC KNTDSRCKAR QLELNERTCR CDKPRR
Purity >95% as determined by SDS-PAGE and HPLC.
MW 19.3 kDa
Source E. coli
Expression Region 27-191aa
Activity Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using human umbilical vein endothelial cells(HUVEC) is between 1.0-8.0 ng/ml.
Uniprot ID P15692
Product Description

Recombinant human VEGFA active protein

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