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Catalog Number | orb80115 |
---|---|
Category | Proteins |
Description | Human VEGF protein |
Target | Human VEGF |
Conjugation | Unconjugated |
Buffer/Preservatives | The protein was lyophilized from concentrated (1mg/ml) solution with no additives. |
Purity | > 98.0% as determined by RP-HPLC and analysis by SDS-PAGE |
Protein Sequence | The sequence of the first five N-terminal amino acids was determined and was found to be Ala-Pro-Met-Ala-Glu. |
Tested applications | FA, HPLC, SDS-PAGE |
Application notes | < strong>Experiment Notes: Protein quantitation was carried out by two independent methods1. UV spectroscopy at 280 nm using the absorbency value of 0.2875 as the extinction coefficient for 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). 2. Analysis by RP-HPLC, using calibrated solution of VEGF as Reference Standard. |
Biological Origin | E.coli |
Biological Activity | Determined by the dose-dependent stimulation of the proliferation of human umbilical vein endothelial cells (HUVEC) using concentration range of 1.0-8.0 ng/ml. |
Solubility (25°C) | It is recommended to reconstitute the lyophilized Vascular Endothelial Growth Factor in sterile H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions. |
Storage | Store at 4°C for up to two weeks. For long term storage, aliquot and store at -20°C, avoid freeze/thaw cycles. |
Alternative names | Vascular endothelial growth factor A protein, Vasc Read more... |
Note | For research use only |
ELISA, FC, ICC, IF, IHC, IHC-Fr, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
FC, ICC, IF, IHC, IHC-Fr, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
FC, ICC, IHC, WB | |
Human | |
Rabbit | |
Polyclonal | |
Unconjugated |
The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining. | |
The protein has a predicted molecular mass of 14.8 kDa after removal of the signal peptide. | |
Mammalian |