Human VEGF protein

• Catalog Number: orb80115
• Category: Proteins
• Description: Human VEGF protein
• Tested applications: FA, HPLC, SDS-PAGE
• Purity: > 98.0% as determined by RP-HPLC and analysis by SDS-PAGE
• Conjugation: Unconjugated
• Target: Human VEGF
• Solubility (25°C): It is recommended to reconstitute the lyophilized Vascular Endothelial Growth Factor in sterile H₂O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.
• Protein Sequence: The sequence of the first five N-terminal amino acids was determined and was found to be Ala-Pro-Met-Ala-Glu.
• Biological Origin: E.coli
• Biological Activity: Determined by the dose-dependent stimulation of the proliferation of human umbilical vein endothelial cells (HUVEC) using concentration range of 1.0-8.0 ng/ml.
• Storage: Store at 4°C for up to two weeks. For long term storage, aliquot and store at -20°C, avoid freeze/thaw cycles.
• Buffer/Preservatives: The protein was lyophilized from concentrated (1mg/ml) solution with no additives.
• Alternative names: Vascular endothelial growth factor A protein, Vasc
• Note: For research use only
• Application notes: < strong>Experiment Notes: Protein quantitation was carried out by two independent methods1. UV spectroscopy at 280 nm using the absorbency value of 0.2875 as the extinction coefficient for 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). 2. Analysis by RP-HPLC, using calibrated solution of VEGF as Reference Standard.
• Expiration Date: 6 months from date of receipt.