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Human VEGF protein

Human VEGF protein

Catalog Number: orb80115

DispatchUsually dispatched within 2-3 weeks
$ 340.00
Catalog Numberorb80115
CategoryProteins
DescriptionHuman VEGF protein
TargetHuman VEGF
ConjugationUnconjugated
Buffer/PreservativesThe protein was lyophilized from concentrated (1mg/ml) solution with no additives.
Purity> 98.0% as determined by RP-HPLC and analysis by SDS-PAGE
Protein SequenceThe sequence of the first five N-terminal amino acids was determined and was found to be Ala-Pro-Met-Ala-Glu.
Tested applicationsFA, HPLC, SDS-PAGE
Application notes< strong>Experiment Notes: Protein quantitation was carried out by two independent methods1. UV spectroscopy at 280 nm using the absorbency value of 0.2875 as the extinction coefficient for 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). 2. Analysis by RP-HPLC, using calibrated solution of VEGF as Reference Standard.
Biological OriginE.coli
Biological ActivityDetermined by the dose-dependent stimulation of the proliferation of human umbilical vein endothelial cells (HUVEC) using concentration range of 1.0-8.0 ng/ml.
Solubility (25°C)It is recommended to reconstitute the lyophilized Vascular Endothelial Growth Factor in sterile H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.
StorageStore at 4°C for up to two weeks. For long term storage, aliquot and store at -20°C, avoid freeze/thaw cycles.
Alternative namesVascular endothelial growth factor A protein, Vasc
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NoteFor research use only
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