Human VEGF-D Protein

• Catalog Number: orb257928
• Category: Proteins
• Description: Vascular endothelial growth factor D (VEGF-D) is also known as C-fos induced growth factor (FIGF), which belongs to the PDGF / VEGF growth factor family and is active in angiogenesis, lymphangiogenesis, and endothelial cell growth, stimulating their proliferation and migration and also has effects on the permeability of blood vessels. This secreted protein VEGF-D / FIGF undergoes a complex proteolytic maturation, generating multiple processed forms that bind and activate VEGFR-2 and VEGFR-3. The structure and function of this protein is similar to those of VEGFC. FIGF / VEGF-D is highly expressed in lung, heart, small intestine and fetal lung. FIGF / VEGF-D may function in the formation of the venous and lymphatic vascular systems during embryogenesis, and also in the maintenance of differentiated lymphatic endothelium in adults. Binds and activates VEGFR-2 (KDR / FLK1) and VEGFR-3 (FLT4) receptors.
• Reactivity: Human
• Tag: C-9×His
• Form/Appearance: Powder
• Purity: 95%
• Conjugation: Unconjugated
• MW: 13.4 kDa
• Target: VEGF-D
• Source: Human VEGF-D, His Tag (orb257928) is expressed from human 293 cells (HEK293). It contains AA Phe 93 - Ser 201 (Accession # AAH27948).
• Expression System: HEK293
• Biological Activity: Human VEGF R3, Fc Tag captured on CM5 chip via Anti-human IgG Fc antibodies surface can bind Human VEGF-D, His Tag with an affinity constant of 19.7 nM as determined in a SPR assay (Biacore T200) (Routinely tested).
• Expression Region: Phe 93 - Ser 201
• Endotoxins: 1.0 EU per μg
• NCBI: AAH27948
• Storage: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.
• Buffer/Preservatives: PBS, pH7.4
• Alternative names: FIGF,VEGFD
• Note: For research use only
• Application notes: This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 13.4 kDa. The protein migrates as 19-22 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Expiration Date: 6 months from date of receipt.

SDS-PAGE analysis of Human VEGF-D protein

Human VEGF R3, Fc Tag (Cat. No. orb18510) captured on CM5 chip via Anti-human IgG Fc antibodies surface can bind Human VEGF-D, His Tag (Cat. No. orb257928) with an affinity constant of 19.7 nM as determined in a SPR assay (Biacore T200) (Routinely tested).