Human TRAP protein

• Catalog Number: orb80099
• Category: Proteins
• Description: sCD40 Human Recombinant produced in E.coli is non-glycosylated, Polypeptide chain containing 149 amino acids and having a molecular mass of 16308 Dalton. The sCD40 is purified by proprietary chromatographic techniques.
• Tested applications: FA, HPLC, SDS-PAGE
• Purity: > 98.0% as determined by RP-HPLC and analysis by SDS-PAGE
• Conjugation: Unconjugated
• Target: sCD40L
• Solubility (25°C): It is recommended to reconstitute the lyophilized sCD40 in sterile H₂O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.
• Protein Sequence: The sequence of the first five N-terminal amino acids was determined and was found to be Met-Gln-Lys-Gly-Asp.
• Biological Activity: The ED50 as determined by the dose-dependant stimulation of IL-12 induction IL-8 production by human PBMC was found to be 5-10 ng/ml.
• Storage: Store at 4°C for up to two weeks. For long term storage, aliquot and store at -20°C, avoid freeze/thaw cycles.
• Buffer/Preservatives: Lyophilized from sterile concentrated solution (1mg/ml) with 10mM Sodium Phosphate pH=7.5.
• Alternative names: CD40-L protein, Tumor necrosis factor ligand super
• Note: For research use only
• Application notes: Experiment Notes: Protein quantitation was carried out by two independent methods1. UV spectroscopy at 280 nm using the absorbency value of 1.1 as the extinction coefficient for 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). 2. Analysis by RP-HPLC, using standard solution of CD40 as Reference Standard.
• Expiration Date: 6 months from date of receipt.