Human TNF alpha protein

• Catalog Number: orb80107
• Category: Proteins
• Description: Human TNF alpha protein
• Tested applications: FA, HPLC, SDS-PAGE
• Purity: > 98.0% as determined by RP-HPLC and analysis by SDS-PAGE
• Conjugation: Unconjugated
• Target: Human TNF alpha
• Solubility (25°C): It is recommended to reconstitute the lyophilized Tumor Necrosis Factor-alpha in sterile H₂O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.
• Protein Sequence: The sequence of the first five N-terminal amino acids was determined and was found to be Met-Val-Arg-Ser-Ser.
• Biological Activity: The ED50 as determined by the cytolysis of murine L929 cells in the presence of Actinomycin D, corresponding to Specific Activity of 50,000,000 IU/mg.
• Storage: Store at 4°C for up to two weeks. For long term storage, aliquot and store at -20°C, avoid freeze/thaw cycles.
• Buffer/Preservatives: 1mg of TNF-alpha Human contain 20 mM PBS, pH-7.2, and 10mM NaCl.
• Alternative names: APC1 protein, Cachectin protein, DIF protein, Diff
• Note: For research use only
• Application notes: Experiment Notes: Protein quantitation was carried out by two independent methods1. UV spectroscopy at 280 nm using the absorbency value of 1.234 as the extinction coefficient for 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). 2. Analysis by RP-HPLC, using calibrated solution of TNF-a as Reference Standard.
• Expiration Date: 6 months from date of receipt.

SDS-PAGE analysis of Human TNF alpha protein