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Catalog Number | orb180447 |
---|---|
Category | Proteins |
Description | Recombinant human TNF alpha protein |
Tag | C-His |
Form/Appearance | Lyophilized from a 0.2 um filtered solution of 20mM Tris-HCl,150mMNaCl,pH8.0. |
UniProt ID | P01375 |
Protein Sequence | Val77-Leu233 |
Source | E.coli |
Expression System | E. coli |
Endotoxins | Less than 0.1 ng/ug (1 EU/ug) |
Storage | Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 4-7°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months. |
Buffer/Preservatives | Lyophilized from a 0.2 μm filtered solution of 20mM PB,150mM NaCl,pH7.4. |
Alternative names | APC1 protein, Cachectin protein, DIF protein, Diff Read more... |
Note | For research use only |
Application notes | 6His tag at the C-terminus. |
Expiration Date | 6 months from date of receipt. |
Greater than 85% as determined by SDS-PAGE. | |
19.4 kDa | |
E.coli |
Greater than 90% as determined by SDS-PAGE. | |
19.4 kDa | |
Yeast |
Unconjugated | |
95% | |
17.4 kDa | |
Human TNF-alpha, premium grade (orb257883) is expressed from human 293 cells (HEK293). It contains AA Val 77 - Leu 233 (Accession # P01375-1). |
Unconjugated | |
95% | |
18.3 kDa | |
Human TNF-alpha, His Tag (active trimer) (MALS verified) (orb383503) is expressed from human 293 cells (HEK293). It contains AA Val 77 - Leu 233 (Accession # NP_000585.2). |
Greater than 95% as determined by SDS-PAGE. | |
17.5 kDa | |
E.coli |
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