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Catalog Number | orb754179 |
---|---|
Category | Proteins |
Description | Recombinant human TIMP2 protein |
Tested applications | ELISA, WB |
Reactivity | Human |
Tag | N-terminal His Tag |
Purity | Greater than 95% as determined by SDS-PAGE |
MW | 21.2 kDa |
Formula | Lyophilized from a 0.2 μm filtered solution in 10 mM Hepes, 500 mM NaCl with 5% trehalose, pH7.4 |
UniProt ID | P16035 |
Source | E.Coli |
Expression System | 27-220 |
Expression Region | 27-220 |
Storage | -20°C for 12 months as lyophilized;2-8°C for 1 month under sterile conditions after reconstitution |
Buffer/Preservatives | Lyophilized from a 0.2 μm filtered solution in 10 mM Hepes, 500 mM NaCl with 5% trehalose, pH7.4 |
Alternative names | CSC 21K, CSC-21K, CSC21K, Metalloproteinase inhibi Read more... |
Note | For research use only |
Application notes | Centrifuge the vial at 10,000 rpm for 1 minute, reconstitute at 200 μg/ml in sterile distilled water by gentle pipetting 2-3 times, don’t vortex |
Expiration Date | 6 months from date of receipt. |
Unconjugated | |
95% | |
21.5 kDa | |
Human TIMP1, His Tag (orb257869) is expressed from human 293 cells (HEK293). It contains AA Cys 24 - Ala 207 (Accession # P01033-1). |
Unconjugated | |
95% | |
22.6 kDa | |
Human TIMP-2, His Tag (orb257870) is expressed from human 293 cells (HEK293). It contains AA Cys 27 - Pro 220 (Accession # NP_003246.1). |
Greater than 90% as determined by SDS-PAGE. | |
48.5 kDa | |
E.coli |
ELISA, MS, SDS-PAGE, WB | |
Greater than 95% as determined by SEC-HPLC and reducing SDS-PAGE. | |
Human cells |
> 95% by Tris-Bis PAGE, > 95% by SEC-HPLC | |
KMP2120, Recombinant Human TIMP-2 Protein is produced by Expi293 expression system. The target protein is expressed with sequence (Cys27-Pro220) of Human TIMP-2 fused with a His Tag at the C-terminal. |
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