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Catalog Number | orb428803 |
---|---|
Category | Proteins |
Description | Recombinant of human TGFA protein |
Form/Appearance | Sterile Filtered White lyophilized (freeze-dried) powder. |
Purity | Greater than 95.0% as determined by SDS-PAGE. |
Solubility (25°C) | It is recommended to reconstitute the lyophilized TGFA in sterile 18M-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions. |
Protein Sequence | VVSHFNDCPD SHTQFCFHGT CRFLVQEDKP ACVCHSGYVG ARCEHADLLA |
Source | Escherichia Coli |
Biological Activity | The ED50, as measured in a proliferation assay using mouse BALB/c 3T3 cells, is 0.395ng/ml. |
Storage | Stability: Lyophilized TGFA although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution TGFA should be stored at 4°C between 2-7 days and for future use below -18°C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles |
Buffer/Preservatives | Lyophilized from a 0.2µm filtered solution in 0.1% TFA. |
Alternative names | Transforming Growth Factor Alpha, Protransforming Read more... |
Note | For research use only |
Application notes | Cytokines And Growth Factors |
Expiration Date | 6 months from date of receipt. |
The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining. | |
The protein has a predicted molecular mass of 34.1 kDa after removal of the signal peptide. The apparent molecular mass of TGFA-hFc is approximately 35-55 kDa due to glycosylation. | |
Mammalian |
98.00% | |
32.9 kDa (predicted). Due to glycosylation, the protein migrates to 38-45 kDa based on Tris-Bis PAGE result. |
94.90% | |
5.6 kDa (predicted); 13 kDa (reducing conditions) |
ELISA, WB | |
Greater than 95% as determined by SDS-PAGE | |
26.2 kDa | |
E.Coli |