Human TFPI Protein

• Catalog Number: orb257863
• Category: Proteins
• Description: Tissue factor pathway inhibitor (TFPI) is also known as Extrinsic pathway inhibitor (EPI), Lipoprotein - associated coagulation inhibitor (LACI), is a plasma proteinase inhibitor synthesized by vascular endothelial cells and part of it is associated with glycosaminoglycans of these cells. TFPI is a single-chain polypeptide which can reversibly inhibit Factor Xa (Xa) and Thrombin (Factor IIa). TFPI is a secreted protein with a N­terminal acidic region, three Kunitz (K) domains separated with by two linker regions, and a C­terminal basic region. The first K domain inhibits coagulation factor VIIa complexed to tissue factor (TF); The second K domain inhibits factor Xa; The third K domain binds to heparin; The C­terminal basic region may have several functions. For example, it plays an important role in binding of TFPI to cell surfaces.
• Reactivity: Human
• Tag: C-6×His
• Form/Appearance: Powder
• Purity: 90%
• Conjugation: Unconjugated
• MW: 30.0 kDa
• Target: TFPI
• Protein Sequence: NP_006278.1
• Source: Human TFPI, His Tag (orb257863) is expressed from human 293 cells (HEK293). It contains AA Asp 29 - Lys 282 (Accession # NP_006278.1).
• Expression System: HEK293
• Expression Region: Asp 29 - Lys 282
• Endotoxins: 1.0 EU per μg
• NCBI: NP_006278.1
• Storage: -20℃
• Buffer/Preservatives: PBS, pH7.4
• Alternative names: TFPI,LACI,TFPI1,EPI,TFI
• Note: For research use only
• Application notes: This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 30.0 kDa. The protein migrates as 41-45 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Expiration Date: 6 months from date of receipt.

SDS-PAGE analysis of Human TFPI protein