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Catalog Number | orb419320 |
---|---|
Category | Proteins |
Description | Recombinant Human SPARC active |
Tag | Tag-Free |
Form/Appearance | Lyophilized powder |
Purity | > 98% as determined by SDS-PAGE and HPLC. |
MW | 32.7 kDa |
UniProt ID | P09486 |
Protein Sequence | APQQEALPDETEVVEETVAEVTEVSVGANPVQVEVGEFDDGAEETEEEVVAENPCQNHHCKHGKVCELDENNTPMCVCQDPTSCPAPIGEFEKVCSNDNKTFDSSCHFFATKCTLEGTKKGHKLHLDYIGPCKYIPPCLDSELTEFPLRMRDWLKNVLVTLYERDEDNNLLTEKQKLRVKKIHENEKRLEAGDHPVELLARDFEKNYNMYIFPVHWQFGQLDQHPIDGYLSHTELAPLRAPLIPMEHCTTRFFETCDLDNDKYIALDEWAGCFGIKQKDIDKDLVI |
Protein Length | Partial |
Source | E.Coli |
Expression System | Expression Region: 18-303aa. Protein Length: Partial |
Biological Activity | Fully biologically active when compared to standard. The ED50 as determined by its ability to inhibit the cell growth of Mv1Lu mink lung epithelial cells is less than 3.0 µg/mL, corresponding to a specific activity of > 333 IU/mg. |
Expression Region | 18-303aa |
Endotoxins | Less than 1.0 EU/µg as determined by LAL method. |
Storage | The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃. |
Buffer/Preservatives | Lyophilized from a 0.2 µm filtered PBS, pH 7.4 |
Alternative names | Basement-membrane protein 40, Osteonectin, ON, Sec Read more... |
Note | For research use only |
Application notes | Tag Info: NO-taggedExpression Region: 18-297aaSequence Info: Full Length of Isoform PlGF-2 |
Expiration Date | 6 months from date of receipt. |
SDS-PAGE analysis of Human SPRC protein
> 95% as determined by SDS-PAGE and HPLC. | |
36.1 kDa | |
E.Coli |
Human | |
62.5 ng/mL-4000 ng/mL | |
15.6 ng/mL |
ELISA, WB | |
Greater than 95% as determined by SDS-PAGE | |
30.7 kDa | |
E.Coli |
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