HUMAN-SHB(Y268) Antibody

• Catalog Number: orb1788356
• Category: Antibodies
• Description: Purified Rabbit Polyclonal Antibody (Pab)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, WB
• Predicted Reactivity: Mouse
• Reactivity: Human
• Isotype: Rabbit IgG
• Immunogen: 250-290 aa
• Antibody Type: Primary Antibody
• Dilution range: WB: 1:1000, FC: 1:25
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Conjugation: Unconjugated
• MW: 55042 Da
• Target: This antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 250-290 amino acids from human.
• UniProt ID: Q15464
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: SH2 domain-containing adapter protein B, SHB
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Flow cytometric analysis of HepG2 cells using Phospho-HUMAN-SHB (Y268). ctrl (green) compared to an isotype control of rabbit IgG (blue). Diluted at 1:25 dilution. An Alexa Fluor 488 goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody.

Western blot analysis of lysates from HepG2, Jurkat, K562 cell line (from left to right), using Phospho-HUMAN-SHB (Y268).ctrl. Diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 20ug per lane.