Human SDF 1 beta protein

• Catalog Number: orb80161
• Category: Proteins
• Description: Human SDF1 beta protein
• Tested applications: FA, HPLC, SDS-PAGE
• Purity: > 98.0% as determined by RP-HPLC and analysis by SDS-PAGE
• Conjugation: Unconjugated
• Target: Human SDF1 beta
• Solubility (25°C): It is recommended to reconstitute the lyophilized Stromal Cell-Derived Factor-1b in sterile H₂O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.
• Protein Sequence: The sequence of the first five N-terminal amino acids was determined and was found to be Lys-Pro-Val-Ser-Leu.
• Biological Activity: The specific activity as determined by its ability to chemoattract human peripheral cells activated with PHA and IL-2 using concentation of 20-80 ng/ml.
• Storage: Store at 4°C for up to two weeks. For long term storage, aliquot and store at -20°C, avoid freeze/thaw cycles.
• Buffer/Preservatives: The CXCL12 protein was lyophilized from concentrated (1mg/ml) sterile solution containing no additives.
• Alternative names: C-X-C motif chemokine 12 protein, CXCL12 protein,
• Note: For research use only
• Application notes: Experiment Notes: Protein quantitation was carried out by two independent methods: 1. UV spectroscopy at 280 nm using the absorbency value of 1.06 as the extinction coefficient for 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). 2. Analysis by RP-HPLC, using standard solution of SDF-1 beta as Reference Standard.
• Expiration Date: 6 months from date of receipt.

SDS-PAGE analysis of Human SDF 1 beta protein