Human PCSK9 Protein, His Tag

• Catalog Number: orb257737
• Category: Proteins
• Description: Human PCSK9, His Tag is expressed from human 293 cells (HEK293). It contains AA Gln 31 - Gln 692 (Accession # Q8NBP7-1).
• Target: PCSK9
• Conjugation: Unconjugated
• Tag: C-10×His
• Reactivity: Human
• Form/Appearance: Powder
• Buffer/Preservatives: PBS, pH 7.4
• Purity: 90%
• UniProt ID: Q8NBP7
• MW: 13.8 kDa and 59.2 kDa
• Application notes: This protein carries a polyhistidine tag at the C-terminus. This protein undergoes autocatalytic cleavage to release the pro-peptide and mature chain. The pro-peptide and mature chain are associated through non‑covalent interactions and with a calculated MW of 13.8 kDa and 59.2 kDa respectively. The protein migrates as 17 kDa and 65-70 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Expression System: HEK293
• Endotoxins: 1.0 EU per μg
• Expression Region: Gln 31 - Gln 692
• Storage: -20°C to -70°C for 24 months in lyophilized state;. -70°C for 24 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower
• Research Area: Disease Biomarkers
• Note: For research use only
• Expiration Date: 6 months from date of receipt.

SDS-PAGE analysis of Human PCSK9 protein

Line graph illustrates about the sensitivity range of Human PCSK9 protein using different concentrations