Human Noggin protein

• Catalog Number: orb80092
• Category: Proteins
• Description: Human Noggin proteinÂ
• Tested applications: FA, HPLC, SDS-PAGE
• Purity: > 95.0% as determined by SDS-PAGE.
• Conjugation: Unconjugated
• Target: Noggin
• Solubility (25°C): It is recommended to be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in 10mM HAc to concentration of 0.1-1.0 mg/mL. Further dilutions should be made in appropriate buffered solutions.
• Protein Sequence: MQHYLHIRPA PSDNLPLVDL IEHPDPIFDP KEKDLNETLL RSLLGGHYDP GFMATSPPED RPGGGGGAAG GAEDLAELDQ LLRQRPSGAM PSEIKGLEFS EGLAQGKKQR LSKKLRRKLQ MWLWSQTFCP VLYAWNDLGS RFWPRYVKVG SCFSKRSCSV PEGMVCKPSK SVHLTVLRWR CQRRGQRCGW IPIQYPIISE CKCSC.
• Biological Activity: The ED50 was determined by its ability to inhibit 5.0 ng/ml of BMP-4 induced alkaline phosphatase production by ATDC-5 chondrogenic cells. The expected ED50 for this effect is 0.05-0.08 ug/ml of Noggin.
• Storage: Store at 4°C for up to two weeks. For long term storage, aliquot and store at -20°C, avoid freeze/thaw cycles.
• Buffer/Preservatives: Lyophilized from 0.2 um filtered solution in 30% CH3CN, 0.1% TFA.
• Alternative names: Nog Protein, NOGG_HUMAN Protein, Noggin Protein
• Note: For research use only
• Application notes: Experiment Notes: Protein quantitation was carried out by two independent methods1. UV spectroscopy at 280 nm using the absorbency value of 1.76 as the extinction coefficient for 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). 2. Analysis by RP-HPLC, using standard solution of Noggin as Reference Standard.
• Expiration Date: 6 months from date of receipt.