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Human Noggin protein
Catalog Number: orb80092
| Catalog Number | orb80092 |
|---|---|
| Category | Proteins |
| Description | Human Noggin protein |
| Tested applications | FA, HPLC, SDS-PAGE |
| Purity | > 95.0% as determined by SDS-PAGE. |
| Conjugation | Unconjugated |
| Target | Noggin |
| Solubility (25°C) | It is recommended to be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in 10mM HAc to concentration of 0.1-1.0 mg/mL. Further dilutions should be made in appropriate buffered solutions. |
| Protein Sequence | MQHYLHIRPA PSDNLPLVDL IEHPDPIFDP KEKDLNETLL RSLLGGHYDP GFMATSPPED RPGGGGGAAG GAEDLAELDQ LLRQRPSGAM PSEIKGLEFS EGLAQGKKQR LSKKLRRKLQ MWLWSQTFCP VLYAWNDLGS RFWPRYVKVG SCFSKRSCSV PEGMVCKPSK SVHLTVLRWR CQRRGQRCGW IPIQYPIISE CKCSC. |
| Biological Origin | E.coli |
| Biological Activity | The ED50 was determined by its ability to inhibit 5.0 ng/ml of BMP-4 induced alkaline phosphatase production by ATDC-5 chondrogenic cells. The expected ED50 for this effect is 0.05-0.08 ug/ml of Noggin. |
| Storage | Store at 4°C for up to two weeks. For long term storage, aliquot and store at -20°C, avoid freeze/thaw cycles. |
| Buffer/Preservatives | Lyophilized from 0.2 um filtered solution in 30% CH3CN, 0.1% TFA. |
| Alternative names | Nog Protein, NOGG_HUMAN Protein, Noggin Protein Read more... |
| Note | For research use only |
| Application notes | < strong>Experiment Notes: Protein quantitation was carried out by two independent methods1. UV spectroscopy at 280 nm using the absorbency value of 1.76 as the extinction coefficient for 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). 2. Analysis by RP-HPLC, using standard solution of Noggin as Reference Standard. |
| Expiration Date | 6 months from date of receipt. |
- Item 1 of 1
Human Noggin Protein, Fc Tag [orb257715]
Unconjugated
95%
49.2 kDa
Human Noggin Protein, Fc Tag, premium grade (orb257715) is expressed from human 293 cells (HEK293). It contains AA Gln 28 - Cys 232 (Accession # Q13253-1).
100 μg, 1 mg, 20 μg - Item 1 of 1
Human Noggin (C-Fc) Protein [orb1471746]
Greater than 95% as determined by reducing SDS-PAGE.
50.2 KDa
Mammalian
10 μg, 50 μg - Item 1 of 1
Recombinant human Noggin protein (HEK293) [orb1516599]
> 90% as determined by SDS PAGE
23 kDa
100 μg, 500 μg Noggin/NOG Protein, Mouse, Recombinant (His) [orb1961257]
> 99.9%
30 KDa (reducing condition)
1 mg, 500 μg, 10 μg, 50 μgRecombinant Human Noggin protein(NOG) (Active) [orb1650599]
1 mg, 5 μg, 20 μg, 100 μg, 250 μg, 500 μg
