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Catalog Number | orb223844 |
---|---|
Category | Proteins |
Description | Recombinant Human Midkine protein |
Tested applications | SDS-PAGE |
Reactivity | Human |
Purity | > 97% by SDS-PAGE and HPLC analyses. |
Conjugation | Unconjugated |
MW | 13.4 kDa |
Target | Human Midkine |
Source | E. coli |
Biological Activity | Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human neutrophils is in a concentration range of 0.1-10 ng/ml. |
Storage | Store at 4°C for up to two weeks. For long term storage, aliquot and store at -20°C, avoid freeze/thaw cycles. |
Buffer/Preservatives | Sterile Filtered White lyophilized (freeze-dried) powder. |
Note | For research use only |
Expiration Date | 6 months from date of receipt. |
SDS-PAGE analysis of Human Midkine protein
> 97 % by SDS-PAGE and HPLC analyses. | |
Approximately 13.4 kDa, a single non-glycosylated polypeptide chain containing 123 amino acids. | |
Escherichia coli |
The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining. | |
The protein has a predicted molecular mass of 39.6 kDa after removal of the signal peptide. The apparent molecular mass of MDK-hFc is approximately 35-55 kDa due to glycosylation. | |
Mammalian |
The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining. | |
The protein has a predicted molecular mass of 39.4 kDa after removal of the signal peptide. The apparent molecular mass of cMDK-hFc is approximately 35-55 kDa due to glycosylation. | |
Mammalian |
Greater than 90% as determined by SDS-PAGE. | |
40.4 kDa | |
E.coli |