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Catalog Number | orb426881 |
---|---|
Category | Proteins |
Description | Recombinant of human,yeast LIF protein |
Form/Appearance | Sterile Filtered White lyophilized (freeze-dried) powder. |
Purity | Greater than 98.0% as determined by:(a) Analysis by RP-HPLC.(b) Analysis by SDS-PAGE. |
Solubility (25°C) | It is recommended to reconstitute the lyophilized LIF in sterile 18M-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions. |
Protein Sequence | S P L P I T P V N A T C A I R H P C H N N L M N Q I R S Q L A Q L N G S A N A L F I L Y Y T A Q G E P F P N N L D K L C G P N V T D F P P F H A N G T E K A K L V E L Y R I V V Y L G T S L G N I T R D Q K I L N P S A L S L H S K L N A T A D I L R G L L S N V L C R L C S K Y H V G H V D V T Y G P D T S G K D V F Q K K K L G C Q L L G K Y K Q I I A V L A Q A F |
Source | Pichia pastoris |
Biological Activity | The biological activity of recombinant human LIF was measured by the ability to induce differentiation of murine M1 myeloid leukemic cells. The minimal detectable concentration of human LIF in this assay is 1 x 108 units/mg. |
Storage | Stability: Lyophilized LIF although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution LIF should be stored at 4°C between 2-7 days and for future use below -18°C. Please prevent freeze-thaw cycles |
Buffer/Preservatives | The protein was lyophilized from a 0.2 µm filtered PBS. |
Alternative names | CDF, HILDA, D-FACTOR, Differentiation- stimulating Read more... |
Note | For research use only |
Application notes | Cytokines And Growth Factors |
Expiration Date | 6 months from date of receipt. |
> 98% as determined by SDS-PAGE and HPLC. | |
19.7 kDa | |
E.Coli |
Unconjugated | |
95% | |
115.5 kDa | |
Human LIF R, Fc Tag (orb257674) is expressed from human 293 cells (HEK293). It contains AA Gln 45 - Ser 833 (Accession # P42702-1). |
Unconjugated | |
90% | |
19.9 kDa | |
Human LIF Protein, premium grade (orb257990) is expressed from human 293 cells (HEK293). It contains AA Ser 23 - Phe 202 (Accession # AAH69540.1). |
Greater than 95% as determined by SDS-PAGE. | |
19.7 kDa | |
E.coli |