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Human Keratinocyte Growth Factor protein
Catalog Number: orb80085
| Catalog Number | orb80085 |
|---|---|
| Category | Proteins |
| Description | Keratinocyte Growth Factor-1 Human Recombinant produced in E.coli is a single, non-glycosylated, polypeptide chain containing 164 amino acids and having a molecular mass of 18995 Dalton.The FGF-7 is purified by proprietary chromatographic techniques. |
| Tested applications | FA, HPLC, SDS-PAGE |
| Purity | > 98.0% as determined by RP-HPLC and analysis by SDS-PAGE |
| Conjugation | Unconjugated |
| Target | KGF |
| Solubility (25°C) | It is recommended to reconstitute the lyophilized Keratinocyte Growth Factor in sterile H2O not less than 100 μg/ml, which can then be further diluted to other aqueous solutions. |
| Protein Sequence | The sequence of the first five N-terminal amino acids was determined and was found to be Met-Cys-Asn-Asp-Met. |
| Biological Activity | The ED50, calculated by the dose-dependant stimulation of KGF-responsive BaF3 indicator cells (measured by 3H-thymidine uptake) is 10ng/ml corresponding to specific activity of 100,000 Units/mg. Biorbyt's Keratinocyte Growth Factor biological activity was measured by the KGF's mitogenic activity on BaF3 cells expressing the KGF receptor. |
| Storage | Store at 4°C for up to two weeks. For long term storage, aliquot and store at -20°C, avoid freeze/thaw cycles. |
| Buffer/Preservatives | The protein was lyophilized from concentrated (1mg/ml) solution with no additives. |
| Alternative names | HBGF-7 protein, FGF7 protein, FGF-7 protein, KGF p Read more... |
| Note | For research use only |
| Application notes | Experiment Notes: Protein quantitation was carried out by two independent methods 1. UV spectroscopy at 280 nm using the absorbency value of 0.9 as the extinction coefficient for 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). 2. Analysis by RP-HPLC, using calibrated solution of KGF as Reference Standard. |
| Expiration Date | 6 months from date of receipt. |
SDS-PAGE analysis of Human Keratinocyte Growth Factor protein
- Item 1 of 1
Human FGF7 protein (Active) [orb359153]
> 96% as determined by SDS-PAGE and HPLC.
18.9 kDa
E.Coli
10 μg, 100 μg, 500 μg 
Human FGF7 protein [orb604094]
Greater than 90% as determined by SDS-PAGE.
32.2 kDa
E.coli
20 μg, 100 μg, 1 mg- Item 1 of 1
Human FGFR2c protein [orb668920]
Functional Studies, IA, WB
> 95%
41.2
HEK293 cells
100 μg, 50 μg, 10 μg - Item 1 of 1
Human FGFR2b protein [orb668919]
Functional Studies, IA, WB
> 95%
41.2
HEK293 cells
100 μg, 50 μg, 10 μg Human FGF10 protein [orb358540]
Greater than 90% as determined by SDS-PAGE.
35.4 kDa
E.coli
20 μg, 100 μg, 1 mg
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