Human Interferon-alpha 2a protein

• Catalog Number: orb80038
• Category: Proteins
• Description: Interferon Alpha Human 2a Recombinant produced in E.coli is a single, non-glycosylated, polypeptide chain containing 165 amino acids and having a molecular mass of 19241 Dalton.The Interferon-a 2a gene was obtained from human leukocytes.The IFN-A 2a is purified by proprietary chromatographic techniques.
• Tested applications: FA, HPLC, SDS-PAGE
• Purity: > 98.0% as determined by both(a) Analysis by RP-HPLC.(b) Analysis by SDS-PAGE.
• Conjugation: Unconjugated
• Target: IFN 2a
• Solubility (25°C): It is recommended to reconstitute the lyophilized Interferon-alpha 2a in sterile H₂O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.
• Protein Sequence: The sequence of the first five N-terminal amino acids was determined and was found to be Cys-Asp-Leu-Pro-Gln, conforming to the sequence of native human IFN-a.N-terminal methionine has been completely removed enzymatically.
• Biological Origin: E.coli
• Biological Activity: The specific activity as determined in viral resistance assay using bovine kidney MDBK cells was found to be 270,000,000 IU/mg.
• Storage: Store at 4°C for up to two weeks. For long term storage, aliquot and store at -20°C, avoid freeze/thaw cycles.
• Buffer/Preservatives: Lyophilized lyophilized without additives.
• Alternative names: Leukocyte interferon protein, cell interferon prot
• Note: For research use only
• Application notes: < strong>Experiment Notes: Protein quantitation was carried out by two independent methods1. UV spectroscopy at 280 nm using the absorbency value of 0.924 as the extinction coefficient for 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). 2. Analysis by RP-HPLC, using calibrated solution of IFN-a 2a as Reference Standard.
• Expiration Date: 6 months from date of receipt.

SDS-PAGE analysis of Human Interferon-alpha 2a protein