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Product Overview
Product Name Human Insulin ELISA kit
Catalog Number orb78858
Range 4 uIU/ml - 300 uIU/ml
Sensitivity 4 uIU/ml
Tested applicationsELISA
Target Insulin ELISA kit. Human
Alternative Names
Product Properties
Storage Store at 4°C. For long term storage aliquot and store at -20°C or -80°C. Avoid repeated freeze/thaw cycles.
Note For research use only.
Kit Components
  • Pre-Coated 96-well Strip Microplate
  • Wash Buffer
  • Assay Diluent(s)
  • Biotinylated Detection Antibody
  • HRP-Streptavidin
  • TMB One-Step Substrate
  • Stop Solution
  • Lyophilized Standard
Product Description

Human Insulin ELISA Kit for Serum, Plasma, Cell Culture Supernatants and Urine.

Application Notes
Protocol details
  • » Prepare all reagents, samples and standards as instructed in the manual.
  • » Add 100 μl of standard or sample to each well.
  • » Incubate 2.5 h at RT or O/N at 4°C.
  • » Add 100 μl of prepared biotin antibody to each well.
  • » Incubate 1 h at RT.
  • » Add 100 μl prepared streptavidin solution to each well.
  • » Incubate 45 min at RT.
  • » Add 100 μl TMB One-Step Substrate Reagent to each well.
  • » Incubate 30 min at RT.
  • » Add 50 μl Stop Solution to each well.
  • » Read plate at 450 nm immediately.
Additional Documents
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