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Human IL4 protein
Catalog Number: orb80061
| Catalog Number | orb80061 |
|---|---|
| Category | Proteins |
| Description | Human IL4 protein |
| Tested applications | FA, HPLC, SDS-PAGE |
| Purity | > 98.0% as determined by RP-HPLC and analysis by SDS-PAGE |
| Conjugation | Unconjugated |
| Target | Human IL4 |
| Solubility (25°C) | It is recommended to reconstitute the lyophilized Interleukin-4 in sterile H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions. |
| Protein Sequence | The sequence of the first five N-terminal amino acids was determined and was found to be Met-His-Lys-Cys-Asp. |
| Biological Origin | E.coli |
| Biological Activity | The ED50 as determined by the dose-dependant stimulation of TF-1 cells is 0.2 ng/ml, corresponding to Specific Activity of 5x1,000,000 IU/mg. |
| Storage | Store at 4°C for up to two weeks. For long term storage, aliquot and store at -20°C, avoid freeze/thaw cycles. |
| Buffer/Preservatives | Lyophilized from concentrated (1mg/ml) solution in water containing no additives. |
| Alternative names | B cell growth factor 1 protein, B cell IgG differe Read more... |
| Note | For research use only |
| Application notes | < strong>Experiment Notes: Protein quantitation was carried out by two independent methods 1. UV spectroscopy at 280 nm using the absorbency value of 0.594 as the extinction coefficient for 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). 2. Analysis by RP-HPLC, using standard solution of IL-4 as Reference Standard. |
| Expiration Date | 6 months from date of receipt. |
SDS-PAGE analysis of Human IL4 protein
- Item 1 of 1
Human IL4 protein (Active) [orb358970]
> 98% as determined by SDS-PAGE and HPLC.
15.0 kDa
E.Coli
5 μg, 100 μg, 500 μg - Item 1 of 2
Human IL-4 Protein [orb257598]
Unconjugated
90%
15.0 kDa
Human IL-4, premium grade (orb257598) is expressed from human 293 cells (HEK293). It contains AA His 25 - Ser 153 (Accession # P05112-1).
1 mg, 50 μg, 20 μg - Item 1 of 1
Human IL4 protein [orb594794]
Greater than 95% as determined by SDS-PAGE.
15.1 kDa
E.coli
10 μg, 50 μg, 1 mg, 500 μg - Item 1 of 1
Human IL4 protein [orb594802]
Greater than 95% as determined by SDS-PAGE.
14.97 kDa
Mammalian cell
10 μg, 50 μg, 1 mg, 500 μg - Item 1 of 1
Human IL4 protein [orb594805]
Greater than 95% as determined by SDS-PAGE.
16 kDa
Mammalian cell
1 mg, 500 μg, 10 μg, 50 μg
