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Human IL2 protein
Catalog Number: orb80055
| Catalog Number | orb80055 |
|---|---|
| Category | Proteins |
| Description | IL2 Human Recombinant produced in E.coli is a single, non-glycosylated mutein (variant form) of human IL-2 polypeptide chain containing 134 amino acids and having a molecular mass of 15517 Dalton. Our Interleukin -2 has an Ser substitute for Cysteine at position 126.The IL-2 is purified by proprietary chromatographic techniques. |
| Target | IL2 |
| Conjugation | Unconjugated |
| Buffer/Preservatives | The protein (1.1mg/ml) was lyophilized after extensive dialysis against 0.17mg sodium monobasic 0.89mg dibasic sodium phosphate buffer to pH=7.5. |
| Purity | > 98.0% as determined by RP-HPLC and analysis by SDS-PAGE |
| Protein Sequence | The sequence of the first five N-terminal amino acids was determined and was found to be Met-Ala-Pro-Thr-Ser. |
| Tested applications | FA, HPLC, SDS-PAGE |
| Application notes | < strong>Experiment Notes: Protein quantitation was carried out by two independent methods1. UV spectroscopy at 280 nm using the absorbency value of 0.614 as the extinction coefficient for 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). 2. Analysis by RP-HPLC, using calibrated solution of IL-2 as Reference Standard. |
| Biological Origin | E.coli |
| Biological Activity | The ED50 as determined by the dose-dependant stimulation of murine CTLL-2C ells is 0.0645 ng/ml, corresponding to Specific Activity of 16.9 MIU/mg. |
| Solubility (25°C) | It is recommended to reconstitute the lyophilized Interleukin in sterile 20 mM AcOH not less than 100ug/ml, which can then be further diluted to other aqueous solutions. |
| Storage | Store at 4°C for up to two weeks. For long term storage, aliquot and store at -20°C, avoid freeze/thaw cycles. |
| Alternative names | T-cell growth factor (TCGF) protein, Aldesleukin p Read more... |
| Note | For research use only |
SDS-PAGE analysis of Human IL2 protein
- Item 1 of 1
Human IL2 protein (Active) [orb358968]
> 97% as determined by SDS-PAGE and HPLC.
15.4 kDa
E.Coli
10 μg, 100 μg, 500 μg - Item 1 of 1
Human IL2 protein [orb594792]
Greater than 95% as determined by SDS-PAGE.
15.5 kDa
E.coli
1 mg, 500 μg, 10 μg, 50 μg - Item 1 of 1
Human IL2 protein [orb594804]
Greater than 95% as determined by SDS-PAGE.
16.4 kDa
Mammalian cell
500 μg, 1 mg, 10 μg, 50 μg - Item 1 of 1
Human IL2 protein [orb594825]
Greater than 95% as determined by SDS-PAGE.
16.5 kDa
Mammalian cell
50 μg, 10 μg, 500 μg, 1 mg - Item 1 of 3
Human IL2 protein [orb246752]
Greater than 90% as determined by SDS-PAGE.
17.4 kDa
Yeast
20 μg, 1 mg, 100 μg
