You have no items in your shopping cart.
Cart summary
Item 1 of 1
Human IL15 protein
Catalog Number: orb80076
| Catalog Number | orb80076 |
|---|---|
| Category | Proteins |
| Description | IL15 Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 114 amino acids and having a molecular mass of 12774 Dalton. The IL-15 is purified by proprietary chromatographic techniques. |
| Tested applications | FA, HPLC, SDS-PAGE |
| Purity | > 98.0% as determined by(a) Analysis by RP-HPLC.(b) Analysis SDS-PAGE. |
| Conjugation | Unconjugated |
| Target | IL15 |
| Solubility (25°C) | It is recommended to reconstitute the lyophilized Interleukin-15 in sterile H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions. |
| Protein Sequence | The sequence of the first five N-terminal amino acids was determined and was found to be Asn-Trp-Val-Asn-Val. |
| Biological Origin | E.coli |
| Biological Activity | The ED50 as determined by the dose-dependant stimulation of the proliferation of mouse CTLL-2 was found to be 0.5 ng/ml, corresponding to Specific Activity of 2,000,000 IU/mg. |
| Storage | Store at 4°C for up to two weeks. For long term storage, aliquot and store at -20°C, avoid freeze/thaw cycles. |
| Buffer/Preservatives | The protein was lyophilized from concentrated (1mg/ml) solution with 5mM Tris pH 8.0. |
| Alternative names | IL-15 protein, MGC9721 protein Read more... |
| Note | For research use only |
| Application notes | < strong>Experiment Notes: Protein quantitation was carried out by two independent methods1. UV spectroscopy at 280 nm using the absorbency value of 0.55 as the extinction coefficient for 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). 2. Analysis by RP-HPLC, using calibrated solution of IL-15 as Reference Standard. |
| Expiration Date | 6 months from date of receipt. |
SDS-PAGE analysis of Human IL15 protein
- Item 1 of 1
Human IL15 protein (Active) [orb358961]
> 97% as determined by SDS-PAGE and HPLC.
12.9 kDa
E.Coli
10 μg, 100 μg, 500 μg - Item 1 of 1
Human IL15 protein [orb594793]
Greater than 95% as determined by SDS-PAGE.
12.5 kDa
E.coli
10 μg, 50 μg, 1 mg, 500 μg - Item 1 of 3
Human IL15 Protein, hFc Tag [orb1291067]
The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.
The protein has a predicted molecular mass of 38.9 kDa after removal of the signal peptide. The apparent molecular mass of IL15-hFc is approximately 35-55kDa due to glycosylation.
Mammalian
10 μg, 50 μg, 100 μg - Item 1 of 3
- Item 1 of 3
