Human IL-21 Protein

• Catalog Number: orb545751
• Category: Proteins
• Description: Interleukin-21 (IL-21) is a secreted protein which belongs to the IL-15 / IL-21 family. Interleukin-21 / IL-21 belongs to a family of cytokines that bind to a composite receptor consisting of a private receptor (IL21R) and the common cytokine receptor gamma chain (gamma(C)). Interleukin-21 / IL-21 impacts a number of cell types, including CD8+ memory T cells, NK cells and subsets of CD4 memory T cells. The IL-21R is widely distributed on lympho-haematopoietic cells. IL-21 is a pleiotropic cytokine produced by CD4+ T cells in response to antigenic stimulation. Its action generally enhances antigen-specific responses of immune cells. IL-21 promotes the anti-tumor activity of CD8+ T-cells and NK cells. IL-21 exerts its effect through binding to a specific type I cytokine receptor, IL-21R, which also contains the γ chain (γc) found in other cytokine receptors including IL-2, IL-4, IL-7, IL-9 and IL-15. The IL-21/IL-21R interaction triggers a cascade of events which includes activation of the tyrosine kinases JAK1 and JAK3, followed by activation of the transcription factors STAT1 and STAT3.
• Reactivity: Human
• Tag: C-Fc
• Form/Appearance: Powder
• Purity: 95%
• Conjugation: Unconjugated
• MW: 41.9 kDa
• Target: IL-21
• Source: Human IL-21, Fc Tag (orb545751) is expressed from human 293 cells (HEK293). It contains AA Gln 30 - Ser 162 (Accession # Q9HBE4-1).
• Expression System: HEK293
• Biological Activity: Immobilized Human IL-21 R, His Tag at 2 μg/mL (100 μL/well) can bind Human IL-21, Fc Tag with a linear range of 0.2-8 ng/mL (QC tested).
• Expression Region: Gln 30 - Ser 162
• Endotoxins: 1.0 EU per μg
• NCBI: NP_068575.1
• Storage: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.
• Buffer/Preservatives: 50 mM Tris, 100 mM Glycine, 150 mM NaCl, pH7.5
• Alternative names: IL21,Za11,Interleukin-21
• Note: For research use only
• Application notes: This protein carries a human IgG1 Fc tag at the C-terminus. The protein has a calculated MW of 41.9 kDa. The protein migrates as 45 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Expiration Date: 6 months from date of receipt.

Immobilized Human IL-21 R, His Tag (Cat. No. orb257627) at 2 μg/mL (100 μL/well) can bind Human IL-21, Fc Tag (Cat. No. orb545751) with a linear range of 0.2-8 ng/mL (QC tested).