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Catalog Number | orb80045 |
---|---|
Category | Proteins |
Description | Insulin-Like Growth Factor-I Human Recombinant produced in E.coli is a single, non-glycosylated, polypeptide chain containing 70 amino acids and having a molecular mass of 7655 Dalton. IGF-I is purified by proprietary chromatographic techniques. |
Tested applications | FA, HPLC, SDS-PAGE |
Purity | > 95.0% as determined by(a) Analysis by RP-HPLC(b) Analysis by SDS-PAGE. |
Conjugation | Unconjugated |
Target | IGF1 |
Solubility (25°C) | It is recommended to reconstitute the lyophilized IGF-1 in sterile H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions. |
Protein Sequence | GPETLCGAEL VDALQFVCGD RGFYFNKPTG YGSSSRRAPQ TGIVDECCFR SCDLRRLEMY CAPLKPAKSA. |
Biological Activity | The ED50, calculated by the dose-dependant proliferation of murine BALB\C 3T3 cells (measured by 3H-thymidine uptake) is 1.0 ng/ml, corresponding to specific activity of 1x1,000,000 U/mg. For most in-vitro applications, IGF-I exerts its biological activity in the concentration range of 0.2-20 ng/ml. |
Storage | Store at 4°C for up to two weeks. For long term storage, aliquot and store at -20°C, avoid freeze/thaw cycles. |
Buffer/Preservatives | The protein was lyophilized with no additives. |
Alternative names | Somatomedin C protein, IGF-I protein, IGFI protein Read more... |
Note | For research use only |
Application notes | Experiment Notes: Protein quantitation was carried out by two independent methods 1. UV spectroscopy at 280 nm using the absorbency value of 0.502 as the extinction coefficient for 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). 2. Analysis by RP-HPLC, using calibrated solution of IGF1 as Reference Standard. |
Expiration Date | 6 months from date of receipt. |
SDS-PAGE analysis of Human IGF1 protein
Unconjugated | |
90% | |
30.6 kDa | |
Human IGFBP-3, His Tag (orb1146966) is expressed from human 293 cells (HEK293). It contains AA Gly 28 - Lys 291 (Accession # P17936-1). |
ELISA, FA, HPLC, SDS-PAGE, WB | |
Unconjugated | |
> 95% pure by SDS-PAGE and HPLC analyses. | |
9.1 kDa |
Unconjugated | |
95% | |
129.3 kDa | |
Human IGF-I R, Fc Tag (orb1496214) is expressed from human 293 cells (HEK293). It contains AA Glu 31 - Asn 932 (Accession # P08069-1). |
Unconjugated | |
90% | |
104.8 kDa | |
Human IGF-I R, His Tag (orb511230) is expressed from human 293 cells (HEK293). It contains AA Glu 31 - Asn 932 (Accession # P08069-1). |
> 97% as determined by SDS-PAGE and HPLC. | |
7.4 kDa | |
E.Coli |
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