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Human IGF1 protein

Catalog Number: orb80045

DispatchUsually dispatched within 2-3 weeks
$ 324.00
Catalog Numberorb80045
CategoryProteins
DescriptionInsulin-Like Growth Factor-I Human Recombinant produced in E.coli is a single, non-glycosylated, polypeptide chain containing 70 amino acids and having a molecular mass of 7655 Dalton. IGF-I is purified by proprietary chromatographic techniques.
Tested applicationsFA, HPLC, SDS-PAGE
Purity> 95.0% as determined by(a) Analysis by RP-HPLC(b) Analysis by SDS-PAGE.
ConjugationUnconjugated
TargetIGF1
Solubility (25°C)It is recommended to reconstitute the lyophilized IGF-1 in sterile H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.
Protein SequenceGPETLCGAEL VDALQFVCGD RGFYFNKPTG YGSSSRRAPQ TGIVDECCFR SCDLRRLEMY CAPLKPAKSA.
Biological ActivityThe ED50, calculated by the dose-dependant proliferation of murine BALB\C 3T3 cells (measured by 3H-thymidine uptake) is 1.0 ng/ml, corresponding to specific activity of 1x1,000,000 U/mg. For most in-vitro applications, IGF-I exerts its biological activity in the concentration range of 0.2-20 ng/ml.
StorageStore at 4°C for up to two weeks. For long term storage, aliquot and store at -20°C, avoid freeze/thaw cycles.
Buffer/PreservativesThe protein was lyophilized with no additives.
Alternative namesSomatomedin C protein, IGF-I protein, IGFI protein
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NoteFor research use only
Application notesExperiment Notes: Protein quantitation was carried out by two independent methods 1. UV spectroscopy at 280 nm using the absorbency value of 0.502 as the extinction coefficient for 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). 2. Analysis by RP-HPLC, using calibrated solution of IGF1 as Reference Standard.
Expiration Date6 months from date of receipt.
Human IGF1 protein

SDS-PAGE analysis of Human IGF1 protein

  • Human IGFBP-3 Protein [orb1146966]

    Unconjugated

    90%

    30.6 kDa

    Human IGFBP-3, His Tag (orb1146966) is expressed from human 293 cells (HEK293). It contains AA Gly 28 - Lys 291 (Accession # P17936-1).

    1 mg, 100 μg
  • Human LR3IGF1 protein [orb82618]

    ELISA,  FA,  HPLC,  SDS-PAGE,  WB

    Unconjugated

    > 95% pure by SDS-PAGE and HPLC analyses.

    9.1 kDa

    1 mg, 20 mg, 5 mg
  • Human IGF-I R / CD221 Protein, Fc Tag [orb1496214]

    Unconjugated

    95%

    129.3 kDa

    Human IGF-I R, Fc Tag (orb1496214) is expressed from human 293 cells (HEK293). It contains AA Glu 31 - Asn 932 (Accession # P08069-1).

    1 mg, 100 μg
  • Human IGF-I R / CD221 Protein [orb511230]

    Unconjugated

    90%

    104.8 kDa

    Human IGF-I R, His Tag (orb511230) is expressed from human 293 cells (HEK293). It contains AA Glu 31 - Asn 932 (Accession # P08069-1).

    1 mg, 100 μg
  • Human IGF1 protein (Active) [orb359162]

    > 97% as determined by SDS-PAGE and HPLC.

    7.4 kDa

    E.Coli

    100 μg
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