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Product Overview
Product Name Human IgE
Catalog Number orb22128
Tested applicationsDOT, IEP
Target Human IgE
Product Properties
Form/Appearance Vial with 0.1 mg Human IgE lyophilized from 0.25 ml PBS (pH 7.2).
Preservatives No preservative added.
Storage This product is shipped lyophilized at ambient temperature. After arrival store at 4°C. Prolonged storage may be at -20°C. A fter opening the vial, the lyophilized content is reconstituted by adding 0.25 ml of sterile distilled water, mixed gently by inversion until complete dissolution is obtained. Allow to stand at ambient temperature for 5-10 minutes to reach equilibrium. Reconstituted solution may be stored frozen. Working dilutions may be prepared by adding the required amount of phosphate buffered saline (PBS), should not be refrozen and preferably used within 24 hours. Repeated thawing and freezing should be avoided. If a slight precipitation occurs upon storage, this should be removed by centrifugation and will not affect the performance of the product.
Note For research use only.
Biological Origin Human myeloma serum
Product Description

Chromatographically purified monoclonal Human IgE kappa. Purified Human IgE may be used as a reference antigen, calibrator, coating protein in a variety of immunoassays including immunodiffusion, immunoelectrophoresis, ELISA, Western blotting, dotimmunobinding assays (DIBA).

Application Notes
Application Notes Experiment Notes: The protein is isolated from serum using affinity chromatography.
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