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Human Ig light chain Lambda antibody

Catalog Number: orb248958

DispatchUsually dispatched within 5-10 working days
$ 413.00
Catalog Numberorb248958
CategoryAntibodies
DescriptionMouse monoclonal antibody to Human Ig light chain Lambda
Species/HostMouse
ClonalityMonoclonal
Clone NumberICO106
Tested applicationsFC, IF
ReactivityHuman
ImmunogenNative purified human Ig light chain Lambda.
Form/AppearanceMouse IgG1. Liquid in PBS, pH 7.3, and 0.02% sodium azide.
ConjugationUnconjugated
SourceMouse
StorageShipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
Buffer/PreservativesMouse IgG1. Liquid in PBS, pH 7.3, and 0.02% sodium azide.
NoteFor research use only
Application notes1. Take 100 μl peripheral blood anticoagulated by EDTA and add to the bottom of 5 ml tube. 2. Add appropriate amount of antibody to the bottom of flow tube mixing with the whole blood; incubate for 30 minutes at room temperature. 3. Add 2 ml RBC lysis buffer; incubate for 10 minutes after mixing; dissolve red blood cells. 4. Sample tube is set to 1000 rpm centrifugation for 5 minutes; discard the supernatant. 5. Add 2 ml PBS wash buffer to resuspend the cells; then 1000 rpm centrifugation for 5 minutes; discard the supernatant. 6. Add appropriate amount of fluorescent-labeled anti-mouse IgGs and incubate for 20 minutes away from light at room temperature. 7. Add 2 ml PBS wash buffer to resuspend the cells; then 1000 rpm centrifugation for 5 minutes; discard the supernatant. 8. Add 0.5 ml PBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 °C then measured).
Expiration Date12 months from date of receipt.
Human Ig light chain Lambda antibody

Flow cytometric analysis of human peripheral blood lymphocytes using human Ig light chain Lambda antibody

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