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Human IDH1 protein

SKU: orb706958

Description

Recombinant Human IDH1 Protein is produced by E.coli expression system and the target gene encoding Met1-Leu414 is expressed with a 8His tag at the C-terminus.

Research Area

Metabolism Research

Images & Validation

Tested ApplicationsELISA, MS, SDS-PAGE, WB
Application Notes
Please minimize freeze-thaw cycles.

Key Properties

SourceE. coli
TargetIDH1
ReactivityHuman
EndotoxinsLess than 0.1 ng/µg (1 IEU/µg) as determined by LAL test.
PurityGreater than 95% as determined by reducing SDS-PAGE.
Protein SequenceMSKKISGGSV VEMQGDEMTR IIWELIKEKL IFPYVELDLH SYDLGIENRD ATNDQVTKDA AEAIKKHNVG VKCATITPDE KRVEEFKLKQ MWKSPNGTIR NILGGTVFRE AIICKNIPRL VSGWVKPIII GHHAYGDQYR ATDFVVPGPG KVEITYTPSD GTQKVTYLVH NFEEGGGVAM GMYNQDKSIE DFAHSSFQMA LSKGWPLYLS TKNTILKKYD GRFKDIFQEI YDKQYKSQFE AQKIWYEHRL IDDMVAQAMK SEGGFIWACK NYDGDVQSDS VAQGYGSLGM MTSVLVCPDG KTVEAEAAHG TVTRHYRMYQ KGQETSTNPI ASIFAWTRGL AHRAKLDNNK ELAFFANALE EVSIETIEAG FMTKDLAACI KGLPNVQRSD YLNTFEFMDK LGENLKIKLA QAKLSLEHHH HHHHH

Storage & Handling

StorageStore it at -20°C to -80°C for one year. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Form/AppearanceLiquid
Buffer/Preservatives0.2 μm filtered solution of 50mM Tris, 200mM NaCl, 10% glycerol, pH 8.0.
Expiration Date6 months from date of receipt.
DisclaimerFor research use only

Alternative Names

PICD; Isocitrate dehydrogenase [NADP] cytoplasmic; IDH; Cytosolic NADP-isocitrate dehydrogenase; IDP; NADP(+)-specific ICDH; Oxalosuccinate decarboxylase

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UniProt Details

No UniProt data available

NCBI Gene Details

No NCBI Gene data available

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Datasheet
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Protocol Information

Protein Handling and Storage Guide
Protein Handling Guide
View Guide
WB
Western Blot (IB, immunoblot)
View Protocol
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
View Protocol
SDS-PAGE
Sodium Dodecyl Sulphate PolyAcrylamide Gel Electrophoresis
View Protocol

Human IDH1 protein (orb706958)

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Available Sizes

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10 μg
$ 380.00
50 μg
$ 1,000.00
500 μg
$ 4,850.00
DispatchUsually dispatched within 5-10 working days
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