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Catalog Number | orb80175 |
---|---|
Category | Proteins |
Description | Human HMGB1 protein |
Target | Human HMGB1 |
Conjugation | Unconjugated |
Buffer/Preservatives | The HMG1 (1 mg/ml) was lyophilized after extensive dialyses against 1x PBS pH-7.4. |
Purity | > 95.0% as determined by RP-HPLC and analysis by SDS-PAGE |
Protein Sequence | MGKGDPKKPR GKMSSYAFFV QTCREEHKKK HPDASVNFSE FSKKCSERWK TMSAKEKGKF EDMAKADKAR YEREMKTYIP PKGETKKKFK DPNAPKRPPS AFFLFCSEYR PKIKGEHPGL SIGDVAKKLG EMWNNTAADD KQPYEKKAAK LKEKYEKDIA AYRAKGKPDA AKKGVVKAEK SKKKEEEEDE EDEEDEEEEE DEEDEDEEED DDDELEHHHH HH. |
Tested applications | SDS-PAGE |
Biological Origin | E.coli |
Solubility (25°C) | It is recommended to reconstitute the lyophilized HMGB1 in sterile H2O not less than 100 ug/ml, which can then be further diluted to other aqueous solutions. |
Storage | Store at 4°C for up to two weeks. For long term storage, aliquot and store at -20°C, avoid freeze/thaw cycles. |
Alternative names | Amphoterin protein, Chromosomal protein, nonhiston Read more... |
Note | For research use only |
SDS-PAGE analysis of Human High-Mobility Group Box 1 protein
> 95% as determined by Tris-Bis PAGE; > 95% as determined by SEC-HPLC | |
Due to glycosylation, the protein migrates to 55-70 kDa based on Tris-Bis PAGE result. |
FC, WB | |
Human, Mouse, Rat | |
Mouse | |
Monoclonal | |
Unconjugated |
Human | |
78 pg/mL-5000 pg/mL | |
19.5 pg/mL |