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Catalog Number | orb759207 |
---|---|
Category | Proteins |
Description | Human HGF Protein, Fc Tag |
Reactivity | Human |
Tag | C-Fc |
Form/Appearance | Powder |
Purity | 90% |
Conjugation | Unconjugated |
MW | 106.1 kDa |
Target | HGF |
UniProt ID | P14210 |
Protein Sequence | NP_000592.3 |
Expression System | HEK293 |
Biological Origin | Human |
Expression Region | Gln 32 - Ser 728 |
Endotoxins | 1.0 EU per μg |
NCBI | NP_000592.3 |
Storage | -20°C to -70°C for 12 months in lyophilized state;. -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower |
Buffer/Preservatives | 50 mM Tris, 100 mM Glycine, 0.5 mM Arginine, 150 mM NaCl, pH 7.5 |
Note | For research use only |
Application notes | This protein carries a human IgG1 Fc tag at the C-terminus. The protein has a calculated MW of 106.1 kDa. The protein migrates as 105-120 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation. |
Expiration Date | 6 months from date of receipt. |
The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining. | |
The protein has a predicted molecular mass of 105.8 kDa after removal of the signal peptide.The apparent molecular mass of HGF-hFc is approximately 100-130 kDa due to glycosylation. | |
Mammalian |
The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining. | |
The protein has a predicted molecular mass of 109.3 kDa after removal of the signal peptide. The apparent molecular mass of HGF(1-728)-hFc is approximately 70-130 kDa due to glycosylation. | |
Mammalian |
ELISA, MS, SDS-PAGE, WB | |
Greater than 95% as determined by reducing SDS-PAGE. | |
Human cells |
Unconjugated | |
90% | |
32.5 kDa (α chain) & 95.3 kDa (β chain) |