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Catalog Number | orb244225 |
---|---|
Category | Proteins |
Description | Recombinant human Glial cell line-derived neurotrophic factor |
Tag | N-terminal 6xHis-SUMO-tagged |
Form/Appearance | Liquid or Lyophilized powder |
Purity | Greater than 90% as determined by SDS-PAGE. |
MW | 31.1 kDa |
UniProt ID | P39905 |
Protein Sequence | SPDKQMAVLPRRERNRQAAAANPENSRGKGRRGQRGKNRGCVLTAIHLNVTDLGLGYETKEELIFRYCSGSCDAAETTYDKILKNLSRNRRLVSDKVGQACCRPIAFDDDLSFLDDNLVYHILRKHSAKRCGCI |
Protein Length | Full Length of Mature Protein |
Source | E.coli |
Biological Origin | Homo sapiens (Human) |
Expression Region | 78-211aa |
Endotoxins | Not test. |
Storage | The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃. |
Buffer/Preservatives | If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. |
Alternative names | GDNF Read more... |
Note | For research use only |
Application notes | Full length of His-SUMO-tag and expression region is 78-211aa |
Expiration Date | 6 months from date of receipt. |
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
Unconjugated | |
90% | |
15.1 kDa | |
Human GDNF Protein, premium grade (orb1675340) is expressed from human 293 cells (HEK293). It contains AA Ser 78 - Ile 211 (Accession # P39905-1). |
> 95% as determined by Tris-Bis PAGE; > 95% as determined by SEC-HPLC | |
Due to glycosylation, the protein migrates to 48-62 kDa based on Tris-Bis PAGE result. |
Greater than 90% as determined by SDS-PAGE. | |
53.8 kDa | |
E.coli |
> 97% as determined by SDS-PAGE and HPLC. | |
15.1 kDa | |
E.Coli |