Human Flt-3 Ligand Protein

• Catalog Number: orb257477
• Category: Proteins
• Description: FMS-like tyrosine kinase 3 ligand (Flt-3 Ligand) is also known as FL, Flt3L and FLT3LG, is an α-helical cytokine that promotes the differentiation of multiple hematopoietic cell lineages. FLT3LG is expressed as a noncovalentlylinked dimer by T cells and bone marrow and thymic fibroblasts. Each 36 kDa chain carries approximately 12 kDa of N- and O- linked carbohydrates. FLT3LG is structurally homologous to stem cell factor (SCF) and colony stimulating facor 1 (CSF-1). FLT3LG acts as a growth factor that increases the number of immune cells by activating the hematopoietic progenitors. It also induces the mobilization of the hematopoietic progenitors and stem cells in vivo which may help the system to kill cancer cells. FLT3LG induces the expansion of monocytes and immature dendritic cells as well as early B cell lineage differentiation. FLT3LG cooperates with IL2, IL6, IL7, and IL15 to induce NK cell development and with IL3, IL7 and IL11 to induce terminal B cell maturation. Animal studies also show FLT3LG to reduce the severity of experimentally induced allergic inflammation. FLT3LG is crucial for steady-state pDC and cDC development. A lack of FLT3L results in low levels of DCs.
• Reactivity: Human
• Tag: C-10×His
• Form/Appearance: Powder
• Purity: 90%
• Conjugation: Unconjugated
• MW: 19.9 kDa
• Target: Flt-3 Ligand
• Source: Human Flt-3 Ligand, His Tag (orb257477) is expressed from human 293 cells (HEK293). It contains AA Thr 27 - Pro 185 (Accession # P49771-1).
• Expression System: HEK293
• Biological Activity: Immobilized Human Flt-3, Fc Tag at 2 μg/mL (100 μL/well) can bind Human Flt-3 Ligand, His Tag with a linear range of 0.1-3 ng/mL (QC tested).
• Expression Region: Thr 27 - Pro 185
• Endotoxins: 1.0 EU per μg
• NCBI: NP_001450.2
• Storage: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.
• Buffer/Preservatives: PBS, pH7.4
• Alternative names: FLT3LG,FL,FLT3L,Flt3 ligand
• Note: For research use only
• Application notes: This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 19.9 kDa. The protein migrates as 28-30 kDa and 31-33 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Expiration Date: 6 months from date of receipt.

SDS-PAGE analysis of Human Flt-3 Ligand protein

Immobilized Human Flt-3, Fc Tag (Cat. No. orb570275) at 2 μg/mL (100 μL/well) can bind Human Flt-3 Ligand, His Tag (Cat. No. orb257477) with a linear range of 0.1-3 ng/mL (QC tested).