Human CD47 Protein

Catalog Number: orb511325

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CategoryProteins
Catalog Numberorb511325
ReactivityHuman
ConjugationUnconjugated
MW15.6 kDa
TargetCD47
Protein SequenceNP_942088
TagC-10×His
SourceHuman CD47, His Tag, low endotoxin (CD7-H522a) is expressed from human 293 cells (HEK293). It contains AA Gln 19 - Pro 139 (Accession # NP_942088).
Biological originHuman
Storage-20℃
Purity95%
Form/AppearancePowder
Buffer/PreservativesPBS, pH7.4
Endotoxin0.01 EU per μg
NoteFor research use only.
Expiration Date6 months from date of receipt.
DescriptionLeukocyte surface antigen CD47 is also known as Antigenic surface determinant protein OA3, Integrin-associated protein (IAP) and Protein MER6. CD47 contains 1 Ig-like V-type (immunoglobulin-like) domain. CD47 is very broadly distributed on normal adult tissues. CD47 has a role in both cell adhesion by acting as an adhesion receptor for THBS1 on platelets, and in the modulation of integrins and plays an important role in memory formation and synaptic plasticity in the hippocampus by similarity. CD47 is the receptor for SIRPA, binding to which prevents maturation of immature dendritic cells and inhibits cytokine production by mature dendritic cells. CD47 Interaction with SIRPG mediates cell-cell adhesion, enhances superantigen-dependent T-cell-mediated proliferation and costimulates T-cell activation.
  1. 2 images
    • Paraffin-embedded rat tongue tissue fixed with 4% paraformaldehyde. Antigen retrieval by boiling with citrate buffer. Blocking buffer is goat serum (37 degrees for 20 min.). CD47 antibody at 1:200 dilution with overnight incubation at 4 degrees
    • Paraffin-embedded rat colitis tissue fixed with 4% paraformaldehyde. Antigen retrieval by boiling with citrate buffer. Blocking buffer is goat serum (37 degrees for 20 min.). CD47 antibody at 1:200 dilution with overnight incubation at 4 degrees
    CD47 antibody [orb4838] Custom conjugation service available

    ELISA, FC, IF, IHC-Fr, IHC-P, WB

    Human, Mouse, Rabbit, Rat

    Rabbit

    Polyclonal

    Unconjugated

    100 μl, 200 μl, 50 μl
  2. 2 images
    • Immunohistochemical staining of paraffin embedded human prostate tissue using CD47 antibody (primary antibody at 1:200)
    • Western blot analysis of jurkat cell lysates using CD47 antibody
    CD47 antibody [orb181722]

    ELISA, IHC-P, WB

    Human

    Rabbit

    Polyclonal

    Unconjugated

    50 μg
  3. 2 images
    • SDS-PAGE analysis of Human CD47 protein
    • Line graph illustrates about the sensitivity range of Human CD47 protein using different concentrations
    Human CD47 Protein [orb257293]

    Human CD47, His Tag (CD7-H5227) is expressed from human 293 cells (HEK293). It contains AA Gln 19 - Pro 139 (Accession # NP_942088).

    15.6 kDa

    95%

    Unconjugated

    50 μg, 1 mg
  4. 2 images
    • SDS-PAGE analysis of Human CD47 protein
    • Line graph illustrates about the sensitivity range of Human CD47 protein using different concentrations
    Human CD47 Protein [orb257295]

    Human CD47, Fc Tag (CD7-H5256) is expressed from human 293 cells (HEK293). It contains AA Gln 19 - Pro 139 (Accession # NP_942088).

    40.4 kDa

    95%

    Unconjugated

    100 μg, 1 mg
  5. 1 image
    • Flow cytometric analysis of porcine peripheral blood using CD47 antibody
    CD47 antibody [orb44051]

    FC, IHC-Fr, WB

    Human, Porcine

    Mouse

    Monoclonal

    0.1 mg