Human AQPEP(Aminopeptidase Q) ELISA Kit

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Product Name Human AQPEP(Aminopeptidase Q) ELISA Kit
Catalog Number orb1146930
ReactivityHuman
Range 0.32-20 ng/mL
Sensitivity 0.124 ng/mL
Alternative Names
Note For research use only.
Sample Types Tissue homogenates, cell lysates and other biological fluids.
Description The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Aminopeptidase Q(AQPEP). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Aminopeptidase Q(AQPEP). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Aminopeptidase Q(AQPEP), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Aminopeptidase Q(AQPEP) in the samples is then determined by comparing the OD of the samples to the standard curve.
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