HTRA3 Antibody

• Catalog Number: orb1269038
• Category: Antibodies
• Description: HTRA3 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IHC-P, WB
• Reactivity: Human, Rat
• Isotype: Rabbit Ig
• Immunogen: This HtrA3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 112-144 amino acids from the N-terminal region of human HtrA3.
• Concentration: batch dependent
• Dilution range: For WB starting dilution is: 1:1000For IHC-P starting dilution is: 1:50~100
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 49 kDa
• Target: HTRA3
• UniProt ID: P83110
• NCBI: P83110
• Storage: Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
• Buffer/Preservatives: Supplied in PBS with 0.09% (W/V) sodium azide.
• Alternative names: Serine protease HTRA3, 3421-, High-temperature req
• Note: For research use only
• Application notes: For WB starting dilution is: 1:1000For IHC-P starting dilution is: 1:50~100
• Expiration Date: 12 months from date of receipt.

Western Blot at 1:1000 dilution + human heart lysate Lysates/proteins at 20 ug per lane.

Antibody is used in Western blot to detect HtrA3 in rat testis tissue lysate.

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. BC = breast carcinoma; HC = hepatocarcinoma.

Formalin-fixed and paraffin-embedded human placenta tissue reacted with HtrA3 antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.