HSP27 antibody

• Catalog Number: orb334746
• Category: Antibodies
• Description: Rabbit polyclonal antibody to HSP27
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IH, WB
• Reactivity: Human, Mouse, Rat
• Immunogen: Recombinant protein corresponding to human HSP27.
• Dilution range: WB: 1:1000-1:2000, IHC-P: 1:200-1:500
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Conjugation: Unconjugated
• Target: HSPB1
• Entrez: 3315
• UniProt ID: P04792
• Source: Rabbit
• Storage: Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
• Buffer/Preservatives: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Alternative names: anti HSP60 antibody, anti 60 kDa heat shock protei
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of HSP27 expression in Hela (A), C2C12 (B), mouse brain (C), rat brain (D) whole cell lysates. (Predicted band size: 22 kD; Observed band size: 27 kD)

Immunohistochemical analysis of HSP27 staining in mouse kidney formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.